宫颈癌术后输注细胞因子诱导的杀伤细胞治疗前后外周血免疫指标的变化  

Changes of immune parameters in cervical cancer patients after CIK cell therapy

作　　者：赵花[1] 刘征丽[2] 许霞[1] ZHAO Hua LIU Zhengli and XU Xia(Department of Clinical Laboratory Department of Gynaecology and Obstetrics, Shaanxi Provincial Corps Hospital of Chinese People＇s Armed Police Force,Xi＇ an 710054)

机构地区：[1]武警陕西总队医院检验科,西安710054 [2]武警陕西总队医院妇产科,西安710054

出　　处：《武警医学》2017年第4期358-360,363,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的观察鳞癌、腺癌、腺鳞癌3种类型宫颈癌根治术后使用细胞因子诱导的杀伤细胞(cytokine induced killer,CIK)治疗前后外周血中免疫指标的变化,为临床使用CIK治疗宫颈癌效果评估提供依据。方法收集我院2014-01至2016-05肿瘤科和妇产科行宫颈癌根治术后患者98例(其中鳞癌56例,腺癌25例,腺鳞癌17例)CIK治疗前1 d和治疗1个周期后的外周血各5 ml,收集的外周血按CIK细胞治疗流程进行血清分离,分离后的血清用流式细胞计数仪对血清中CD3^+、CD4^+、CD8^+、CD4/CD8、NK细胞的比例进行检测分析。结果 (1)98例宫颈癌根治术后使用CIK细胞治疗前后外周血中免疫指标的对比显示CD3^+、NK细胞的比例均升高,CD4^+、CD4/CD8比例仅在鳞癌和腺癌中升高,CD8+治疗前后变化不明显。(2)以CD3^+和NK细胞为例可以看出使用CIK细胞治疗后腺癌免疫指标较鳞癌、腺鳞癌有显著提升(P<0.05),鳞癌治疗前后,CD3^+:(60.71±5.84)/(67.27±3.04),NK细胞:(8.92±3.64)/(12.93±6.25);腺癌治疗前后,CD3^+:(59.91±2.49)/(68.32±8.24),NK细胞:(9.04±5.31)/(17.87±6.46);腺鳞癌治疗前后,CD3^+:(59.01±5.90)/(65.23±7.07),NK细胞:(8.59±3.97)/(15.07±2.71)。结论 CIK细胞治疗可以显著提升宫颈癌根治术后患者的免疫功能,对延长生存期,提高生存质量具有重要意义。Objective To observe the changes of immune parameters of patients with different cervical cancer treated with cy- tokine-induced killer（CIK） cells after radical hysterectomy in order to provide data for clinical evaluation. Methods 50 ml peripheral blood was collected the day before CIK cell therapy and after a course of treatment from ninety-eight radical hysterectomy patients （56 cases of squamous carcinoma, 25 cases of adenocarcinoma, and 17 cases of adenosquamous carcinoma） treated in the Department of Gynaecology and Obstetrics and Department of Oncology between January 2014 and September 2016. According to the process of CIK cell therapy, the serum was separated, and the proportion of CD3 ＋ ,CD4＋ ,CD8 ＋ ,CD4/CD8 ,and NK cells in the serum was analyzed using flow cytometry （FCM）. Results 1. The proportion of CD3 ~ and NK cells in the 98 patients was increased after CIK cell thera- py. The proportion of CD4 ＋ and CD4/CD8 rose only in patients of squamous carcinoma and adenocarcinoma. The proportion of CD8 ＋ did not change significantly. 2. The result of CD3＋ and NK cells suggested that after treatment the immune parameters in patients of ad- enocarcinoma were significantly higher than those in patients of squamous carcinoma and adenosquamous carcinoma （ P 〈 0.05 ）. After treatment, CD3 ＋ and NK cells increased from 60.71 ±5.84 to 67.27 ± 3.04 and from 8.92 ± 3.64 to 12.93 ± 6.25 respectively in patients of squamous carcinoma, from 59.91 ± 2.49 to 68.32 ± 8.24 and from 9.04 ± 5.31 to 17.87 ± 6.46 respectively in patients of adenocarcinoma, and finally from 59.01 ± 5.90 to 65.23 ±7.07 and from 59±3.97 to 15.07± 2.71 respectively in patients of adeno- squamous carcinoma. Conclusions CIK cell therapy can markedly enhance the immunity of patients after radical hysterectomy. It＇ s of great significance for improving the quality of survival.

关 键 词：细胞因子诱导的杀伤细胞 宫颈癌 免疫指标 

分 类 号：R737.3[医药卫生—肿瘤]