YM155对骨肉瘤细胞系F5M2增殖和凋亡的影响  

The effect of YM155 on proliferation and apoptosis of osteosarcoma F5M2 cell line

作　　者：陈洁[1] 彭诗元 贠喆[1] 马琼[1] 文艳华[1] 裘秀春[1] 周勇[1] 

机构地区：[1]第四军医大学唐都医院骨肿瘤研究所,陕西西安710038 [2]第四军医大学唐都医院妇产科,陕西西安710038

出　　处：《现代肿瘤医学》2017年第14期2203-2206,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81272441)

摘　　要：目的:探讨YM155对人骨肉瘤细胞系F5M2的作用及其机制。方法:体外培养人骨肉瘤细胞系F5M2,不同浓度YM155处理人骨肉瘤细胞系F5M2,用MTT法检测其对细胞增殖的影响;流式细胞仪检测细胞凋亡率;实时荧光定量PCR检测survivin mRNA的表达;蛋白免疫印迹法检测survivin、caspase-3蛋白的表达。结果:YM155可抑制人骨肉瘤细胞F5M2的增殖,且呈剂量依赖性。随着YM155浓度的升高,人骨肉瘤细胞F5M2凋亡率明显增加,同时能够降低survivin在mRNA水平和蛋白水平的表达,以及激活caspase-3。结论:YM155能够有效抑制人骨肉瘤细胞F5M2增殖,并诱导其凋亡,其可能机制为下调骨肉瘤细胞系F5M2survivin的表达,继而激活caspase凋亡信号通路。Objective：To explore the effect of YM155 on human osteosarcoma cell line F5M2 and its associatedmechanism. Methods ： Human osteosarcoma cell line F5M2 was cultured in vitro and treated with different concentra-tions of YM155. Cell proliferation inhibition rate was measured by MTT assay. The percentage of cell apoptosis was de-tected by flow cytometry. The mRNA level of survivin was detected by Real - time quantitative PCR. The protein level of survivin and caspase -3 was detected by Western blot. Results ： YM155 inhibited F5M2 cell proliferation in a dose -dependent manner. The percentage of F5M2 cell apoptosis evidently increased with YM155 concentration, accompa-nied by decreasing mRNA and protein level of survivin and increasing caspase -3 expression. Conclusion： YM 155 could effectively inhibit the proliferation of human osteosarcoma cell line F5M2 and induce its apoptosis through downregulating survivin expression and activating caspase - 3 pathway.

关 键 词：骨肉瘤 YM155 SURVIVIN 增殖 凋亡 

分 类 号：R738.1[医药卫生—肿瘤]