血清中CD44v5及c-met检测对食管鳞癌高危人群的筛检价值  

The detection of CD44v5 and c-met in serum for screening of high-risk population of esophageal squamous cell carcinoma

作　　者：范志勤[1] 吴振华[1] 王兴名 王勇涛[1] 王洪江[1] 

机构地区：[1]新疆医科大学附属肿瘤医院胸外科,新疆乌鲁木齐830011

出　　处：《现代肿瘤医学》2017年第14期2250-2255,共6页Journal of Modern Oncology

基　　金：新疆维吾尔自治区青年科技创新人才培养工程资助项目(编号:2014721045);新疆维吾尔自治区自然科学基金资助项目(编号:2016D01C342)

摘　　要：目的:检测食管鳞癌患者CD44v5及c-met表达情况,并分析其在癌组织及癌旁组织的表达差异;探讨采用ELISA法检测两者在血清中含量用于食管鳞癌筛检的价值。方法:采用RT-PCR技术和WesternBlot法分别检测CD44v5及c-met基因在食管鳞癌组织及癌旁组织中的表达;并采用酶联免疫吸附法(ELISA法)测定CD44v5及c-met蛋白在100例食管鳞癌患者及60例健康体检者血清的含量。结果:CD44v5、c-met mRNA在食管鳞癌组织中IA值(0.7 3 7±0.1 1 6,0.7 4 8±0.1 2 2)高于相应癌旁食管组织(0.140±0.065,0.147±0.064),差异均具有统计学意义(P<0.01);CD44 v5及c-met蛋白相对表达量在癌组织(1.099±0.230,1.054±0.227)中亦高于相应癌旁组织(0.265±0.105,0.245±0.097)(P<0.01);CD44v5蛋白在食管鳞癌患者血清中的含量[(31.308±10.123)μg/L]高于健康体检者[(19.364±1.680)μg/L],差异具有统计学意义(P<0.01),c-met蛋白在食管鳞癌患者血清中的含量[(101.183±15.335)μg/L]亦高于健康体检者[(56.165±8.979)μg/L](P<0.01)。CD44v5与c-met在食管鳞癌患者血清中的含量具有相关性(r=0.880,P<0.01)。ELISA法检测CD44v5、c-met、二者并联及串联诊断食管鳞癌的ROC曲线下面积分别为0.865、1.000、0.800及0.950,灵敏度分别为91.0%、99.0%、1 0 0.0%、9 0.0%,特异度分别为60.0%、100.0%、60.0%、100.0%。结论:CD44v5与c-met的高表达与食管鳞癌的发生有关,二者具有相关性;ELISA法检测血清中c-met蛋白含量,并联合CD44v5蛋白检测,或许有助于在食管鳞癌高发地区对高危人群进行大规模筛检。Objective: To detect the expression of CD44v5 and c-met in esophageal squamous cell carcinoma( ESCC),and analyze the difference between the carcinoma tissues and adjacent tissues. Explore value of screening for ESCC by detecting the contents of CD44v5 and c-met using ELISA method. Methods: To detect the expression of CD44v5 and c-met in ESCC tissues and its corresponding adjacent non-carcinomatous tissues by RT-PCR and Western-Blot,and use enzyme-linked immunosorbent assay( ELISA) to detect the proteins in serum of 100 ESCC patients and 60 healthy subjects. Results: CD44v5 and c-met mRNA IA value in ESCC tissues( 0. 737 ±0. 116,0. 748 ± 0. 122) were both higher than that of esophageal tissues adjacent to carcinoma( 0. 140 ± 0. 065,0. 147 ±0. 064)( P < 0. 01). The content of CD4 4 v5 and c-met proteins in cancerous tissues( 1. 099 ± 0. 230,1. 054 ±0. 227) were higher than tissues adjacent to carcinoma( 0. 2 6 5 ± 0. 1 0 5,0. 2 4 5 ± 0. 0 9 7)( P < 0. 0 1) respectively. And in serum,CD4 4 v5 and c-met proteins in patients with ESCC [( 31. 308 ± 10. 123) μg/L,( 101. 183 ± 15. 335) μg/L]were higher than healthy subjects [( 19. 364 ± 1. 680) μg/L,( 56. 165 ± 8. 979) μg/L]respectively,and the differences were all statistically significant( P < 0. 01). There was some correlation between CD44v5 expression and c-met in patients with ESCC( r = 0. 880,P < 0. 01). The AUC of the ROC curves of diagnosis of ESCC using ELISA method to detect CD44v5,c-met,both in parallel and series were 0. 865,1. 000,0. 800 and0. 950 respectively. The sensitivity of the diagnosis were 91. 0%,99. 0%,100. 0% and 90. 0% respectively,and the specificity were 60. 0%,100. 0%,60. 0% and 100. 0% respectively. Conclusion: The high expression of CD44v5 and c-met is associated with the occurrence of ESCC,and there's correlation between them. Using ELISA method to detect the contents of c-met in serum,and combining with CD44v5 protein detection,may contribute to mass screening of ESCC in high-risk groups in the areas of prone to ESCC.

关 键 词：食管肿瘤 鳞状细胞癌 CD44V5 C-MET 酶联免疫吸附测定 

分 类 号：R735.1[医药卫生—肿瘤]