小鼠肝组织NTCP表达与肝纤维化程度相关性的研究  

Study on the expression of sodium taurocholate cotransporting polypeptide (NTCP) in mice liver and the correlation with the severity of fibrosis

作　　者：许虎峰[1] 陈孟瑶[1] 孙晨阳[1] 刘琳[2,3] 李海玉[1] 刘天会[2,3] 

机构地区：[1]首都医科大学附属北京友谊医院实验中心,北京100050 [2]首都医科大学附属北京友谊医院肝病中心,北京100050 [3]首都医科大学附属北京友谊医院肝硬化转化医学北京市重点实验室,北京100050

出　　处：《临床和实验医学杂志》2017年第11期1041-1044,共4页Journal of Clinical and Experimental Medicine

基　　金：国家自然基金资助项目(编号:81641022);北京市卫生系统高层次卫生技术人才项目(2013-3-062);王宝恩肝纤维化研究基金资助项目(编号:CFHPC20161019)

摘　　要：目的观察不同肝纤维化程度小鼠肝组织中Na^+-牛磺胆酸共转运多肽(NTCP)表达水平的变化,探讨NTCP表达与肝纤维化严重程度的相关性。方法经口灌胃方法给予10%CCl4油剂建立小鼠肝纤维化模型;在CCl4给药不同时间点取肝组织进行Masson及天狼猩红染色,判断肝纤维化程度;实时荧光定量PCR方法检测不同程度肝纤维化小鼠肝组织中NTCP mRNA水平;Western blot方法检测不同程度肝纤维化小鼠肝组织中NTCP蛋白表达水平。结果与正常对照小鼠相比,肝纤维化程度评分为F1~F2的小鼠肝组织中NTCP mRNA及蛋白表达的水平均有显著增加;而与肝纤维化程度评分为F1~F2的小鼠相比,肝纤维化程度评分为F3~F4的小鼠肝组织中NTCP mRNA及蛋白的表达水平则显著下降。结论 NTCP的表达在肝纤维化发生早期升高,在肝纤维化晚期降低,其表达水平的动态变化有可能提示肝纤维化的发展进程。Objective To observe the expression of sodium taurocholate cotransporting polypeptide （ NTCP） in mice liver at different ges of fibrosis, and to explore the possible relationship between expression of NTCP and the severity of fibrosis. Methods 10% CC14 in olive oil was administered by gavage to mice to establish the fibrosis model. The portions of liver tissue had been collected at different time points after CCL4 administration, and they were stained by Masson and Sirius Red to identify the severity of fibrosis. Real - time quantitative PCR was performed to detect the mRNA levels of NTCP in the liver at different stages of fibrosis. Western blot was performed to detect the protein levels of NTCP in liver with different stages of fibrosis. Results In comparison with control mice, both the levels of mRNA and protein of NTCP in liver tissue were sig-nificantly increased in mice with fibrosis scores at FI and F2. While, the levels of mRNA and protein of NTCP in liver tissue were decreased in mice with fibrosis scores at F3 and F4 when compared with mice with fibrosis scores at FI and F2. Conclusion The expression of NTCP in liver tissue is significantly increased at the early stage of liver fibrosis, and it is decreased at the late stage of liver fibrosis. The dynamic changes of NT-CP may indicate the progression of liver fibrosis.

关 键 词：小鼠 肝纤维化 Na^+- 牛磺胆酸共转运多肽 

分 类 号：R575.2[医药卫生—消化系统]