microRNAs在胸腺上皮性肿瘤中的差异表达  

Differential expressions of microRNAs in thymic epithelial tumors

作　　者：杜军[1,2] 印洪林[1] 周晓军[1] 

机构地区：[1]南方医科大学金陵医院(南京军区南京总医院)病理科,南京210002 [2]安徽省立医院南区病理科

出　　处：《医学研究生学报》2017年第6期619-622,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81372743)

摘　　要：目的微小RNA(microRNAs)在多种肿瘤发生发展中具有重要作用,但在胸腺上皮性肿瘤中的研究较少。文中旨在研究microRNAs在胸腺上皮性肿瘤(B3型胸腺瘤与胸腺癌)中的表达谱及其意义。方法收集南京军区南京总医院病理科2012年1月至2015年1月间的手术切除B3型胸腺瘤3例(对照组)及胸腺鳞状细胞癌3例(病例组),做基因芯片研究。将病例组与对照组数据相比,计算出各miRNA的差异倍数,筛选出上调与下调的miRNA。通过预测网站预测其靶基因,结合文献检索出与胸腺相关的基因。结果 B3型胸腺瘤与胸腺鳞状细胞癌对比发现,32种差异表达miRNAs在胸腺癌中上调,包括miR-125b-1-3p、miR-3175、miR-4462等;19种差异表达miRNAs在胸腺癌中下调,包括miR-361-5p、miR-130a-3p、miR-3651等。miR-377-5p的靶基因包括AKT1、C9、CD19、CDC42、LSS、MYC;miR-485-5p的靶基因包括ADCYAP1R1、ASPA、CAD、CD63等;miR-183-5p的靶基因包括AKAP12、CD28、FOXP1、MDM4等。结论筛选出B3型胸腺瘤与胸腺癌中的差异表达miRNAs,通过预测软件预测这些miRNAs的靶基因,可以为胸腺上皮性肿瘤的进一步治疗提供了有效的依据。Objective MicroRNAs （miRNAs） are of important clinical value in various tumors. However, few studies are reported about their role in thymic epithelial tumors. This article aims to explore differential expression profile of miRNAs in type B3 thymoma and thymie carcinoma. Methods This study included the pathological data about 45 cases of type B3 thymoma and thymic carcinoma surgically treated in our hospital from January 2012 to January 2015, of which 3 cases of type B3 thymoma （control group） and another 3 cases of thymic carcinoma （case group） were subjected to miRNA microarray for determination of the differential expres- sions of miRNAs in the tumor tissues. The up- and down-regulated miRNAs were calculated, their target genes were predicted via on- line databases, and the thymus-related genes were identified. Results Totally, 32 differentially expressed miRNAs （including miR- 125b-1-3p, miR-3175, and miR-4462） were up-regulated and another 19 （including miR-361-Sp, miR-130a-3p, and miR-3651）down-regulated in thymic carcinoma. AKT1, C9, CD19, CDC42, LSS, and MYC were identified as the target genes of miR-377-5p, ADCYAP1R1, ASPA, CAD, and CD63 as the target genes of miR- 458-5p, and AKAP12, CD28, FOXP1, and MDM4 as the target genes of miR-183-5p. Conclusion Differentially expressed miR- NAs were identified in type B3 thymoma and thymic carcinoma and their target genes predicted using the prediction software, which mayprovide some valid evidence for further study of thymic epithelial tumors.

关 键 词：B3型胸腺瘤 胸腺癌 MIRNA芯片 靶基因 

分 类 号：R736.3[医药卫生—肿瘤]