高产期不同产蛋水平蛋鸡肠道微生物群落特征  

The character of the intestinal microbiota associated with laying performance in hens

作　　者：张亚楠[1] 魏单平 韩瑞丽[1] 张彦庭 刘小军[1] 康相涛[1] 王彦彬[1] 

机构地区：[1]河南农业大学牧医工程学院河南省家禽种质资源创新工程研究中心,河南郑州450002 [2]河南省西华县动物卫生监督所,河南西华466600

出　　处：《中国兽医学报》2017年第6期1179-1185,共7页Chinese Journal of Veterinary Science

基　　金：教育部创新团队发展计划资助项目(IRT1236);国家蛋鸡产业技术体系遗传育种岗位专项资金资助项目(CARS-41-K04);河南省重点科技攻关资助项目(152102110058);河南省高等学校重点科研资助项目(16A230011)

摘　　要：为了研究高产期不同产蛋水平蛋鸡肠道微生物多样性特征,探究肠道微生物与蛋鸡产蛋水平之间的关联性。应用16SrRNA基因V4~V5区进行高通量测序,检测高产期3个产蛋水平(高产组/低产组/极低产组:6只/6只/3只)蛋鸡粪便样品微生物的组成与菌群分布。结果表明:厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)、变形菌门(Proteobacteria)在3组中均为优势菌群(≥1%),但各自占比在3组中有所差异;在属水平上,高产组中乳杆菌属丰度(66.05%)是极低产组(20.93%)的3倍;PCoA检测显示不同产蛋水平蛋鸡微生物群落结构差异明显,乳酸杆菌属是不同产蛋水平蛋鸡肠道微生物菌群差异的主要菌属,推测其在肠道微生物中的占比与产蛋水平存在着关联性。In order to study the character of intestinal microbial diversity in laying hens at different stages,and investigate the relationship between intestinal microflora and level of egg laying hens. High-throughput sequencing of V4 V5 variable regions of 16S rRNA genes was utilized to detect the composition and distribution of microorganisms in 3 stages of laying hens （high yield group/ low yield group/very low yield group,n=6 ： 6 ： 3）. The results showed that the Firmicutes, Bacteroidetes Actinobacteria,Proteobacteria were all dominant bacteria in the 3 groups （≥ 1%）, but the proportion in the 3 groups were different. In the genus level, the abundance of genus Lacto- bacillus （66. 05%） in high yield group was 3 times than that in low group （20. 93%）;PCoA showed differences of microbial community structure in different level of laying hens laying obvi- ously,Lactobacilli was predominant specie in different intestinal microbial flora of all levels of egg laying hens,the proportion of which in intestinal microflora assumed to have relation with egg lay- ing performance.

关 键 词：蛋鸡 16S RRNA 微生物群落 高通量测序 

分 类 号：S831[农业科学—畜牧学]