凝胶色谱净化超高效液相色谱法测定食品中10种工业染料  

Simultaneous Determination of Synthetic Dyes in Foods by Ultra Performance Liquid Chromatography Coupled with Gel Permeation Chromatography Clean-up Procedure

作　　者：胡海山[1] 鄢兵[1] 张云伟[1] 龙洲雄 

机构地区：[1]江西省分析测试研究所,江西南昌330029 [2]江西省科技发展研究中心,江西南昌330046

出　　处：《分析科学学报》2017年第3期362-366,共5页Journal of Analytical Science

基　　金：江西省科学技术厅科技计划重点项目(No.20142BBG70032)

摘　　要：建立了测定食品中10种工业染料的超高效液相色谱(UPLC)法。样品经提取后,采用凝胶色谱(GPC)净化后收集浓缩,以乙腈-0.1%甲酸溶液为流动相进行梯度洗脱,10种染料得到良好分离。10种染料线性关系良好,相关系数R^2>0.9992,其检出限为0.011~0.049μg/mL;样品在低、中、高3个加标浓度下,10种染料的回收率为65.0%~107.2%。结果表明该方法适用于食品中10种工业染料的检测,其样品处理方法适用于各类食品,且检测灵敏度高,可实现食品中工业染料的快速测定。A simultaneous detection method of 10 synthetic dyes in foods was developed using ultra performance liquid chromatography. After extraction, the samples were purified by gel permeation chromatography and then concentrated. The sample solution was separated in gradient elution with acetonitrile-0.1% formic acid solution as the mobile phase. The linear relationships were good with the correlation coefficient R^2〉0. 9992. Detection limits were 0. 011-0. 049 μg/mL. The average recoveries for 10 dyes were 65. 0%- 107. 2%. The results indicated that the developed method was rapid and sensitive in determination of synthetic dyes.

关 键 词：超高效液相色谱 凝胶色谱 食品 工业染料 

分 类 号：O657.7[理学—分析化学]