Ⅱ型高尔基体膜蛋白73在肝细胞肝癌中的水平及与肝细胞肝癌预后的相关性  

Serum level of type Ⅱ Golgi protein 73 in hepatocellular carcinoma and its correlation with the prognosis of hepatocellular carcinoma

作　　者：黄飞[1] 贺新祥[1] 刘文俊[1] 杨忠民[1] 王光链[1] 

机构地区：[1]荆州市中心医院,湖北荆州434020

出　　处：《实用预防医学》2017年第7期882-885,共4页Practical Preventive Medicine

摘　　要：目的探究Ⅱ型高尔基体膜蛋白73(GP73)在肝细胞肝癌(hepatocellular carcinoma,HCC)中的水平及与其预后的相关性分析。方法选择本院肝胆外科65例HCC患者和60例健康体检者以及62例单纯性慢性中度乙型肝炎病例(未发生HCC)分别作为病例组、健康对照组和慢性中度乙型肝炎病例组,收集入组者的基本资料以及临床病理资料。ELISA法测定血清(术前)GP73水平,并根据术前血清中GP73水平将病例组中所有HCC患者依据中位数原理进一步分为高GP73组和低GP73组,对比两个亚组之间的肝功能水平、及肿瘤的大小和分级水平。同时用化学发光微粒子免疫分析法(CLEIA)检测血清中AFP的含量,分析AFP和GP73的相关性。随后对术后的HCC患者进行长期随访,记录并统计患者的生存率。结果对比病例组和健康对照组以及慢性中度乙型肝炎患者发现,HCC患者中患乙型肝炎的比例占69.2%,病例组AFP的水平显著高于健康体检组和慢性中度乙型肝炎组(P<0.01)。病例组HCC患者血清中GP73水平较健康对照组显著升高,而慢性中度乙型肝炎患者血清中GP73水平并未表现出显著升高。对比HCC患者中的两个亚组发现,高GP73组HCC患者和低GP73组HCC患者的Child-Pugh肝功能A级、B级、C级的例数以及肿瘤的大小和TNM分级分布差异有统计学意义(P<0.05),高GP73组HCC患者肝脏储备功能较低,且肿瘤的进程较低GP73组患者严重,两组之间在术后60周后生存率之间差异有统计学意义(P<0.05)。分析HCC患者中GP73水平和AFP水平的相关性发现血清中二者水平存在明显的正相关性(r=0.876,P<0.05)。结论 GP73在HCC患者中高表达且GP73的水平和AFP水平呈正相关,和生存率呈负相关,为HCC患者预后的生物标志物之一。Objective To explore the expression level of type U Golgi protein 73 （ GP73 ） in hepatocellular carcinoma （ HCC） and its correlation with the prognosis of HCC. Methods Sixty-five patients with HCC, 60 health check-up individuals and 62 patients with chronic moderate hepatitis B were selected from Department of Hepatobiliary Surgery, Jinzhou Central Hospital to serve as the case group, the healthy control group and the case group of chronic moderate hepatitis B respectively. Their general in- formation and clinical pathological data were collected. ELISA was used to determine the （ preoperative ） serum GP73 level, and then the case group was further divided into the high GP73 subgroup and the low GP73 subgroup according to the preoperative ser- um GP73 level with the median principle. Liver function and tumor size and grading were compared between the two subgroups. Se- rum AFP concentration was detected by particles-based chemiluminescence immunoassay enzyme particles （ CLEIA ） , and the cor- relation between AFP and GP73 was analyzed. A long-term follow-up was conducted in the postoperative HCC patients, and their survival rates were recorded and statistically analyzed. Results A comparison between the case group and the healthy control group, the case group of chronic moderate hepatitis B showed that the proportion of patients with hepatitis B （ 69.2% ） and the AFP levels in the case group were significantly higher than those of the healthy control group and the case group of chronic moderate hepatitis B （both P〈0.01）. The serum GP73 level was significantly higher in the case group than in the healthy control group and the case group of chronic moderate hepatitis B. A comparison between the two subgroups revealed that there were statistically signif- icant differences in the numbers of cases with liver function grades A, B and C based on Child-Pugh grading, the size of the tumor and TNM classification between the high GP73 subgroup and the low GP73 subgroup （ all P〈O.05 ）. Hepati

关 键 词：肝细胞肝癌 Ⅱ型高尔基体膜蛋白73 甲胎蛋白 生存率 

分 类 号：R735.7[医药卫生—肿瘤]