Fra-1基因在喉鳞状细胞癌中的表达研究  

Expression of Fra-1 in laryngeal squamous cell carcinoma

作　　者：张云龙[1] 段云静 宋晓飞[3] 赵瑞力[4] 

机构地区：[1]石家庄市第一医院重症医学三科,石家庄050011 [2]石家庄市第一医院耳鼻喉科 [3]河北省人民医院耳鼻喉科 [4]河北医科大学第四医院耳鼻喉科

出　　处：《临床耳鼻咽喉头颈外科杂志》2017年第12期914-917,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:探讨喉鳞状细胞癌组织中Fra-1基因的表达情况。方法:应用免疫组织化学SP法及RT-PCR检测Fra-1在47例喉鳞状细胞癌组织和21例癌旁正常黏膜中的表达情况,进一步分析此基因在癌组织中表达与各临床病理参数的关系。结果:Fra-1基因在喉鳞状细胞癌组织中蛋白水平阳性表达率均高于癌旁正常黏膜组织(P<0.05),Fra-1基因在喉鳞状细胞癌组织中蛋白水平表达与临床分期、淋巴结转移、吸烟相关,与病理分级、年龄、解剖分区均不相关;Fra-1基因在喉鳞状细胞癌组织中mRNA水平表达量均高于癌旁正常黏膜组织(P<0.01),Fra-1基因在mRNA水平的表达与病理分级、临床分期、淋巴结转移、吸烟相关,与年龄、解剖分区无关。结论:喉鳞状细胞癌组织中Fra-1基因过度表达,此基因可能在喉癌发生、发展过程中发挥作用。Objective：To investigate expressions of Fra-1 in laryngeal squamous cell carcinoma. Method：Using immunohistochemical method and RT PCR detecting 47 cases of laryngeal squamous carcinoma tissues and 21 ca- ses of para-carcinoma tissues with the level of protein and mRNA, we analysed the relationship of the genes ex pression and clinical parameters. Result：The level of protein positive expression of Fra-1 genes in laryngeal squa- mous cancer tissue were higher than adiacent tissues（P〈0.05）. The expression of Fra-1 protein in laryngeal squa- mous cancer tissue were significant correlation with the clinical stages, lymph metastasis, smoking. They had no significant correlation with the pathological grade, age, anatomic zonation. The mRNA level expression of Fra-1 genes in laryngeal squamous cancer tissue were higher than adjacent tissues（P〈0.01）. The expression of Fra-1 gene in the mRNA level was correlated with pathologic stage, clinical stage, lymph metastasis,and smoking, but wasn＇t correlated with age and anatomic zonation. Conclusion： In laryngeal squamous cell carcinoma, the expression of Fra-1 gene are overexpressed. Fra-1 may play a role in the occurrence and development of the laryngeal cancer.

关 键 词：喉肿瘤 癌 鳞状细胞 免疫组织化学 逆转录-多聚酶链反应 Fra-1 

分 类 号：R739.65[医药卫生—肿瘤]