气相色谱法测定红花软胶囊中亚油酸的含量  

Content determination of linoleic acid in safflower soft caplsule by gas chromatograp

作　　者：翟晓茹[1] 李永东[1] 张红兵[1] 张燕[1] ZHAI Xiaoru LI Yongdong ZHANG Hongbing ZHANG Yan(China Institute for Radiation Protection,Taiyuan 030006, Chin)

机构地区：[1]中国辐射防护研究院,山西太原030006

出　　处：《临床医药实践》2017年第6期460-464,共5页Proceeding of Clinical Medicine

基　　金：新疆少数民族医药现代化专项课题(项目编号:200733146-1)

摘　　要：目的:建立红花软胶囊中亚油酸含量的检测方法。方法:色谱柱为固定液88%二氰丙基聚硅氧烷(60 m×0.25 mm,0.20μm),检测器为氢焰离子化检测器,检测器温度280℃,进样口温度250℃,柱温控制采用程序升温,以140℃为起始柱温,保持5 min,以4℃/min升温至240℃,保持15 min。载气为氮气,载气流速:1.0 m L/min,分流比为30∶1。结果:用亚油酸甲酯作对照,其线性范围0.270 5~5.403 3 mg/m L,γ=0.999 8,平均回收率为97.02%(RSD=1.3%,n=9)。结论:该方法准确、灵敏高、重现性好,可用于红花软胶囊中亚油酸含量的测定。Objective ：To establish the gas chromatograph method for quantitative determinate of linoleic acid in safflower soft caplsule. Methods ： A column of 88 % two cyanopropyl polysiloxane （60 m × 0. 25 mm, 0. 20μm） was used. The FID was used as a detector, the temperature of the detector was 280 ℃. The injection port temperature was 250 ℃. The column tempera- ture was controlled by temperature programming to 140 ℃ as a starting column, holded 5 min, with 4℃/min heated to 240 ℃, holded 15min, nitrogen was used an carrier gas, the flow rate was 1.0 mL/min, the splitting ratio was 30： 1. Results：The line- ar range of linoleum acid was O. 270 5 - 5. 4033 mg/mL（r = 0.999 8）, the average recovery was 97.02% （ RSD = 1.3 % , n = 9）. Conclusion ： This GC method was accurate, sensitive and reproducible for the determination of safflower linoleic acid con- tent in soft capsules.

关 键 词：红花软胶囊 气相色谱 亚油酸 

分 类 号：R735.1[医药卫生—肿瘤]