枯草芽孢杆菌发酵生产聚-γ-谷氨酸的条件优化  

Optimization of Fermentation Conditions of Poly-γ-glutamic Acid Production by Bacillus subtilis

作　　者：武国慧 张蕾[1,2] 高德才[1,2] 李鹏程 张鹰 石元亮[1,3] 

机构地区：[1]中国科学院沈阳应用生态研究所,辽宁沈阳110016 [2]中国科学院大学,北京100049 [3]辽宁中科生物工程有限公司,辽宁本溪117004

出　　处：《食品研究与开发》2017年第11期165-170,共6页Food Research and Development

基　　金："十二五"国家科技支撑项目"滨海盐碱地作物稳定性肥料研制与施用技术研究"(2013BAD05B04)

摘　　要：为提高聚-γ-谷氨酸(poly-γ-glutamic acid,γ-PGA)产量,降低其生产成本,利用枯草芽孢杆菌(Bacillus subtilis),采用单因素试验及正交试验优化法,探究培养基组分及发酵条件对γ-PGA发酵产量的影响。结果表明:最佳培养基组成和培养条件为:蔗糖5%,谷氨酸钠6%,氯化铵0.3%,磷酸氢二钾2%,磷酸二氢钾0.1%,硫酸锰0.003%,p H 7.0,接种量为3%,发酵温度33℃,发酵时间48 h。与未优化前γ-PGA产量(15.8 g/L)相比,经优化后的产量达20.8 g/L,提高了31.65%。To improve the production and reduce the cost of γ-PGA, a fermentation study with Bacillus subtilis was conducted to explore the effects of medium compositions and culture conditions on γ-PGA production based on single-factor experiment and orthogonal design. The results showed that the optimized cultivation conditions and medium composition were as follows： sucrose 5 % ; sodium glutamate 6 % ; NH4Cl 0.3 % ; K2HPO4 2 % ; KH2PO4 0.1% ; MnS04 0.003 % ; initial pH 7.0; inoculum amount 3 % ; temperature 33 ℃ ; fermentation time 48 h. In this study, high γ-PGA productivity （20.8 g/L） was obtained under the optimal conditions, 31.65 % higher than the previous conditions （ 15.8 g/L）.

关 键 词：聚-γ-谷氨酸 液体发酵 微生物合成 培养基 正交设计 

分 类 号：TQ922.1[轻工技术与工程—发酵工程]