脂联素对RF/6A细胞增殖、迁移及管腔形成的影响  

Effects of adiponectin on proliferation,migration and tube formation of RF/6A cells

作　　者：谢坤鹏[1] 王新[2] 杜军辉 刘平[2] 

机构地区：[1]河南省立眼科医院(河南省眼科研究所),河南省郑州市450000 [2]郑州人民医院眼科,河南省郑州市450003 [3]西安市第九医院眼科,陕西省西安市710054

出　　处：《眼科新进展》2017年第6期511-514,共4页Recent Advances in Ophthalmology

基　　金：陕西省自然科学基础研究计划面上项目(编号:2016JM8018)~~

摘　　要：目的研究脂联素(adiponectin,APN)对体外培养的猴脉络膜/视网膜内皮细胞(RF/6A)增殖、迁移和管腔形成的影响,探讨APN对脉络膜和视网膜新生血管的抑制作用。方法取体外培养的生长良好的RF/6A细胞用于实验,将细胞随机分为对照组、不同浓度重组APN组(5 pg·mL^(-1)、50 pg·mL^(-1)、500 pg·mL^(-1)预处理1 h)。培养24 h后采用MTT法检测细胞增殖,细胞划痕法检测细胞迁移,基质胶(Matrigel)法检测管腔形成。结果不同浓度重组APN组24 h的细胞增殖均弱于对照组(均为P<0.05),不同浓度重组APN组细胞24 h的迁移面积均小于对照组(均为P<0.05),不同浓度重组APN组管腔形成数均明显少于对照组(均为P<0.05),且细胞增殖、迁移和管腔形成均随着重组APN浓度的升高而降低。结论 APN能够明显抑制RF/6A细胞的血管生成过程,提示APN对于脉络膜和视网膜新生血管具有一定的保护作用。Objective To investigate the effects of adiponectin （APN） on prolif- eration, migration and tube formation of RF/6A cells, and explore the effects of APN on choroidal and retinal angiogenesis. Methods Well cultured RF/6A cells were random- ly divided into the control group and three groups with different concentrations of re- combinant adiponectin （5 pg mL-1 ,50 pg mL-1 ,500 pg mL-1 ） for 1 hour. After 24 hours, cell proliferation, migration and tube formation were detected by MTT assay, wound scratch assay and seeding cells in matrigel, respectively. Resuits The cell pro- liferation in all APN groups was weaker than that of control group （ P 〈 0.05 ）, the cell migration area （pixels） of all APN groups was significantly smaller than that of the con- trol group （P 〈 0.05 ）, and the number of tube formation of all APN groups was signifi- cantly less than the control group （P 〈 0.05 ）. Cell proliferation, migration and tube for- mation decreased along with the increase of APN concentration. Conclusion APN can obviously inhibit the angiogenesis process of RF/6A cells. This inhibitive effect indicates the protective role of APN in choroidal and retinal angiogenesis.

关 键 词：脂联素 血管生成 脉络膜新生血管 视网膜新生血管 

分 类 号：R774.1[医药卫生—眼科]