人参黑斑病菌对人参根系分泌物中氨基酸的化学趋向性响应研究  

Research on chemotaxis response of Alternaria panax to amino acid of ginseng root exudates

作　　者：张爱华[1] 马文丽[1] 雷锋杰[1,2] 安宁波[1] 张欣欣[1] 刘志庆[1] 张连学[1] 

机构地区：[1]吉林农业大学中药材学院吉林省人参工程技术研究中心,吉林长春130118 [2]沈阳农业大学植物保护学院,辽宁沈阳110866

出　　处：《中国中药杂志》2017年第11期2052-2057,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(31100239;31200224);吉林省科技发展计划项目(20140520159JH;20170204018YY)

摘　　要：该实验采用平板对峙培养法和孢子悬滴萌发法探讨了人参黑斑菌对精氨酸、谷氨酸、天冬氨酸以及苏氨酸在不同条件下化学趋向性响应。结果显示,人参黑斑菌对4种氨基酸化学趋向性响应的最优培养温度均为25℃,不同质量浓度和pH的培养条件下人参黑斑菌对4种氨基酸化学趋向性响应有所差异,其中精氨酸、谷氨酸及苏氨酸在质量浓度为2 mg·L^(-1),pH7,天冬氨酸在20 mg·L^(-1),pH 6时,化学趋向性均达到最强,其趋化率分别为1.24,1.38,1.27,1.31;生长速率分别为0.451 0,0.353 0,0.381 3,0.228 8;孢子萌发率分别为57.33%,63%,56.67%,58%;菌丝干重分别为372.9,348.5,314.4,390.2 mg·L^(-1)。氨基酸作为人参根系分泌物中的重要物质对人参黑斑病菌表现出了高效的趋化性效应,有的甚至出现了高浓度抑制的现象。Plate assay and spore germination method were used to study the chemotaxis response of Alternaria panax to arginine, glutamic acid, aspartic acid and threoniue. The result showed that the optimum temperature of A. panax chemotaxis response to four amino acids were all 25 ~C. And chemotaxis responses of A. panax were different under conditions of different concentration and pH value. The chemotaxin reached to the highest under the condition of 2 mg· L- 1 and pH value was 7 for arginine, glutamic acid and threonine while 20 mg · L-1 and pH value was 6 for aspartic acid. The data of chemotactic migration index （CMI） were 1.24, 1.38, 1.27, 1.31 and chemotactic growth rates（CGR） were O. 451 0, 0. 353 , 0. 381 3, 0. 228 8 and spores germination rates（SGR） were 57.33% ,63% ,56.67% ,58% and the dry weight of mycelial （DWM） were 372. 9, 348.5, 314. 4, 390. 2 mg · L-1 respectively. It indicated that the low and middle concentration of amino acid had significant promoting effect on chemotaxis response of A. panax. As important substances generated in ginseng root, amino acids exhibited an efficient chemotaetic effect on A. panax, and some even show inhibition effect under high concentration .

关 键 词：人参黑斑病菌 根系分泌物 氨基酸 化学趋向性 

分 类 号：S435.675[农业科学—农业昆虫与害虫防治]