5-LO在SHR大鼠脑动脉平滑肌细胞中的表达及意义  

Expression and significance of 5-LO in the cerebral artery smooth muscle cells of SHR rats

作　　者：韩晶[1] 杨春林[1] 夏开来[1] 陈少军[1] 

机构地区：[1]三峡大学人民医院神经外科,湖北宜昌443000

出　　处：《西部医学》2017年第6期745-748,共4页Medical Journal of West China

基　　金：湖北省自然科学基金(h2819223)

摘　　要：目的探讨5脂氧合酶(5-LO)在自发性高血压大鼠(SHR)脑动脉平滑肌细胞中的表达及意义。方法选取16周龄雄性SHR和WKY大鼠,各15只,WKY大鼠作为正常对照。采用免疫荧光法检测5-LO在脑动脉血管中的表达,透射电镜观察动脉内膜表现,ELISA检测血清及动脉5-LO表达。结果 WKY大鼠动脉内膜光滑,平滑肌细胞排列整齐无增生,SHR大鼠内皮细胞肿胀,平滑肌细胞肥大变形排列紊乱;SHR大鼠脑动脉平滑肌细胞5-LO荧光强度为(110.93±5.92),明显强于WKY大鼠(P<0.05);SHR大鼠血清和脑动脉5-LO水平分别为(2671.04±30.18)ng/l和(1950.04±30.08)ng/l,明显高于WKY大鼠,差异比较有统计学意义(P<0.05)。结论 5-LO在SHR大鼠脑动脉平滑肌细胞和血液中表达异常增高,可能在高血压脑血管损害过程中起作用。Objective To investigate the expression and significance of 5-lipoxygenase （5-LO） in the cerebral arter- y smooth cells of spontaneously hypertensive rats （SHR）. Methods 16 weeks old male 15 SHR rats and 15 WKY rats were selected in the present study. The expression of 5-LO in cerebral artery was detected by immunofluorescence. Transmission electron microscopy was used to observe the performance of arterial intimal. ELISA was used to detect the expression of 5-LO in serum and artery. Results The artery intimal of WKY rat was smooth. The smooth muscle cells of WKY rat orderly arranged without hyperplasia. The endothelial cell of SHR rat was swelled. The smooth muscle cell of SHR was hypertrophy disorder. The fluorescence intensity of 5-LO in rat cerebral artery smooth muscle cells of SHR rat （110.93±5.92） was significantly stronger than that of WKY rats （P 〈 0.05）. The levels of 5-LO in serum and cerebral artery of SHR rats were significantly higher than that of WKY rats （P 〈 0.05） o Conclusion The expression of 5- LO in rat cerebral artery smooth muscle cells and blood are abnormal increased, which may play a role in the course of hy- pertensive cerebral vascular damage.

关 键 词：5脂氧合酶 自发性高血压大鼠 脑动脉 平滑肌细胞 

分 类 号：R-332[医药卫生] R544.1