响应面法对微拟球藻产微藻蛋白的发酵条件优化  

Optimization of fermentation conditions for protein production of Nannochloropsis gaditana by response surface methodology

作　　者：王怡梅 周鑫鑫[1] 邓刚[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《工业微生物》2017年第3期36-42,共7页Industrial Microbiology

基　　金：国家自然科学基金(No.21006098)

摘　　要：以稳定期微藻蛋白浓度为评价指标,利用响应面设计对微拟球藻(Nannochloropsis gaditana)的分批发酵条件进行优化。在单因素试验的基础上,选取温度、p H、搅拌速度及通气量为影响因子,采用四因素三水平的Box-Benhnken中心组合法设计试验。结果表明:微拟球藻的最佳发酵条件为温度30℃、p H 6.9、搅拌速度340 r/min以及通气量0.65 vvm,在此优化条件下得到微藻蛋白浓度为6.18 g/L,与模型预测值基本相符,较优化前提高了9.18%。In order to improve microalgae protein productivity during stable phase, the response surface methodology method was used to optimize the fermentation conditions of batch cultivation of Nannochloropsis gaditana. Based on the data from single-factor trial, main factors including the temperature, pH, stirring speed and aeration rate were selected as influencing factors, and then the experiments with four factors and three levels were designed according to a Box-Benhnken central composite rule. The results showed that the optimal fermentation conditions were as follows： temperature 30 ℃, pH 6.9, stirring speed 340 r/min and aeration rate 0.65 vvm. Finally, the confirmatory experiment was performed under these optimized conditions. The final protein concentration was as high as 6.18 g/L, increased by 9.18% than before. This result was consistent with the model prediction.

关 键 词：微拟球藻 微藻蛋白 响应面法 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]