自身免疫性疾病患者PD-L1自身抗体的筛查  

Detection of Programmed Death-ligand 1 Autoantibody in Patients with Autoimmune Diseases

作　　者：闫卓红[1] 王小珏[2] 郑小芳[3] 易玲[1] 韦攀健[1] 贾红艳[2] 杨斌[1] 张洪涛[2] 

机构地区：[1]首都医科大学附属北京胸科医院肿瘤免疫室,101149 [2]北京市结核病胸部肿瘤研究所中心实验室,101149 [3]首都医科大学附属北京朝阳医院病理科,100020

出　　处：《结核病与胸部肿瘤》2017年第1期8-11,共4页Tuberculosis and Thoracic Tumor

基　　金：首都卫生发展科研专项（2014-4-2162）;国家自然科学基金（81273209）;“十二五”传染病重大专项（2013ZX10003003）

摘　　要：目的调查自身免疫性疾病和结核病患者PD—L1自身抗体产生情况。方法以重组人PD-L1蛋白为抗原，间接ELISA和免疫印迹法对血清样本进行测定，包括63例类风湿性关节炎（RA）、38例系统性红斑狼疮（SLE）、42例强直性脊柱炎（AS）、28例干燥综合征（ss）、50例结核病（TB）和60例健康人对照。结果ELISA结果显示，与健康人对照组（0．1317±0．0284，N=20）相比，RA（0．1996±0．0579，P〈0．0001）、SLE（0．1689±0．0338，P=0．0005）和AS组（0．1729±0．0619，P=0．0107）PD-LI自身抗体水平显著增高，而SS组（0．1341±0．0397，P=0．6834）无显著性差异。与健康人对照组（0．1541±0．0139，N=40）相比，结核病组（0．1709±0．1069，P=0．4215）PD—L1自身抗体水平无显著差异，但存在ELISA检测阳性个体，经免疫印迹证实存在PD—L1自身抗体。在46％RA、26％SLE、29％AS、1l％SS和12％TB中可检出PD-L1自身抗体，而健康人对照组未检出。结论自身免疫性疾病可检测到PD-L1自身抗体，此为进一步研究PD-L1与免疫相关疾病和免疫检查点调控机理奠定了基础。Objective To screen the presence of PD-L1 autoantibody in patients with autoimmune diseases and tuberculosis. Methods Recombinant human PD-L1 protein was used as detective antigen. PD-L1 autoantibody was analyzed by an enzyme-linked immunosorbent assay （ELISA） and western blot using serum samples from 63 patients with rheumatoid arthritis （RA）, 38 patients with systemic lupus erythematosus （SLE）, 42 patients with ankylosing spondylitis （AS）, 28 patients with Sjogren＇s Syndrome （SS）, 50 patients with tuberculosis （TB） and 60 healthy subjects. Results The level of PD-LI autoantibody detected by ELISA in the patients with RA （0.1996± 0.0579, P〈0.0001）, SLE （0.1689 ± 0.0338,P=0.0005） and AS （0.1729 ± 0.0619, P=0.0107） were significantly higher than that in the healthy subjects （0.1317 ± 0.0284, N=20）, while there was no statistically significant differences in SS group （0.1341 ± 0.0397, P=0.6834）. PD-LI autoantibody level also showed no statistical difference between TB patients （0.1709 ± 0.1069, P=0.4215） and healthy subjects （0.1541 ± 0.0139, N=40）, although some individuals showed a relatively higher level of it by ELISA, which has been verified by western blot. The positive percentages of PD-L1 autoantibody in RA, SLE, AS, SS, TB and healthy subjects were 46%, 26%, 29%, 11%, 12% and 0%, respectively. Conclusion We detected PD-L1 autoantibody in patients with autoimmune diseases, which laid a foundation for further research on the mechanism and regulation of PD-L1 in immune related diseases.

关 键 词：PD-L1 自身抗体 自身免疫性疾病 结核病 ELISA 免疫印迹 

分 类 号：R593.2[医药卫生—内科学]