miR-124调控SOS1表达及对U87胶质瘤细胞增殖的影响  

miR-124 regulates the expression of SOS1 in glioblastoma and has effects on proliferation of U87 glioblastioma cells

作　　者：吕中华[1] 谭春雷[1] 王洪滨[1] 耿建雄 常亮[1] 苏君[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院神经外科,黑龙江哈尔滨150040

出　　处：《现代肿瘤医学》2017年第13期2023-2026,共4页Journal of Modern Oncology

基　　金：黑龙江省青年科学基金(编号:QC2013C093)

摘　　要：目的:探讨miR-124对胶质瘤细胞增殖的抑制作用及作用靶点。方法:应用生物信息整合分析,SOS1是miR-124负向调节胶质瘤细胞的靶点。应用细胞转染和荧光素酶检测分析证实miR-124对SOS1的作用关系。结果:SOS1是miR-124作用靶点,miR-124直接作用于SOS1 mRNA 3'端非编码区(UTR),但不作用于突变型的3'(UTR)。miR-124通过作用于靶点SOS1抑制了体外胶质瘤细胞的增殖。结论:SOS1在恶性胶质瘤细胞中呈正向调控,miR-124的含量上升可导致SOS1含量下降,miR-124通过调节SOS1/Raf/ERK信号通路在抑制细胞生长中起重要作用。Objective：Confirming SOS1 gene is a target of miR - 124 gene regulating glioma cells, miR - 124 can directly act on the 3＂ untranslated region （UTR） of SOS1 mRNA, miR - 124 silence SOS1 inhibition of glioma cell proliferation. Methods：Using bioinformatics integration analysis, SOS1 was the target of miR - 124 negative regulation of glioma cells. Cell transfection and luciferase assay confirmed the role of miR - 124 in SOS1. Results：SOS1 was the target of miR - 124. It was confirmed that miR - 124 directly acted on the 3 ＂untranslated region （UTR） of SOS1 mR- NA,but not on mutant 3＂（UTR）. miR - 124 inhibited the proliferation of glioma cells by acting on target SOS1. Con- dusion ：SOS1 is positively regulated in malignant glioma cells, and our study demonstrates that upregulation miR - 124 is responsible for the downregulation of SOS1 in GBM, and miR - 124 has an important role in inhibiting cell growth by regulating the SOS1/Raf/ERK signaling pathway.

关 键 词：miR-124 SOS1 胶质母细胞瘤 

分 类 号：R730.264[医药卫生—肿瘤]