延龄草总皂苷抑制宫颈癌Hela细胞转移的作用及机制  

Effects of Trillium saponins on metastasis of cervical cancer cells Hela and the possible mechanisms

作　　者：曾小聪 许辉 黄建清 孙阳[2] 邓上飞 杨东晓 袁新荣 

机构地区：[1]解放军第422医院肿瘤科,广东湛江524005 [2]第四军医大学药理教研室,陕西西安710032

出　　处：《现代肿瘤医学》2017年第13期2039-2042,共4页Journal of Modern Oncology

基　　金：湛江市非资助科技攻关计划项目(编号:2016B01017)

摘　　要：目的:观察延龄草总皂苷对宫颈癌Hela细胞侵袭与迁移的影响及其机制。方法:培养宫颈癌细胞Hela,加入延龄草总皂苷(1、2 mg/L)24 h后,采用细胞侵袭实验、划痕实验观察延龄草总皂苷对Hela细胞侵袭与迁移的影响,以Western blot方法检测细胞中MMP-2、MMP-9蛋白的表达。结果:延龄草总皂苷能够有效地抑制Hela细胞的侵袭与迁移,浓度依赖性地降低MMP-2、MMP-9的蛋白表达。结论:延龄草总皂苷可能通过降低MMP-2、MMP-9的表达发挥抑制宫颈癌细胞Hela侵袭与迁移的作用。Objective：To investigate the effects of Trillium saponins on the invasion and migration of cervical cancer cells Hela and explore the possible mechanisms. Methods：Hela cells were treated with different concentrations of Trillium saponins（ 1 or 2 mg/L）. Cell invasion and migration were then evaluated by transwell and wound healing assays. The effect of Trillium saponins on the protein expression of MMPs was investigated by Western blot. Results： Trillium saponins suppressed the invasion and migration of Hela cells significantly. It could reduce the expression of MMP- 2 and MMP- 9 in a concentration dependent manner. Conchlsion：TriHium saponins inhibited Hela metasta- sis, at least in part, by affecting the expression of MMP -2 and MMP -9.

关 键 词：延龄草总皂苷 宫颈癌 侵袭 迁移 MMP-2 MMP-9 

分 类 号：R737.33[医药卫生—肿瘤]