GP73和AFP-L3联合检测在小肝癌诊断中的临床意义  

Significance of combined test of serum GP73 and AFP-L3 in diagnosis of small primary hepatocellular carcinoma

作　　者：赵运胜[1] 高芹 王春华[1] 吴侠[1] 张丽娜[1] 崔辰莹[1] 

机构地区：[1]秦皇岛市第一医院检验科,河北秦皇岛066000 [2]秦皇岛市抚宁区人民医院检验科,河北秦皇岛066300

出　　处：《现代肿瘤医学》2017年第13期2082-2085,共4页Journal of Modern Oncology

摘　　要：目的:评价血清高尔基体蛋白73(GP73)和甲胎蛋白异质体3(AFP-L3)联合检测对低浓度甲胎蛋白(AFP)原发性小肝癌的诊断价值。方法:收集低浓度甲胎蛋白的小肝癌患者50例,非肝癌的消化系统疾病患者80例及健康对照者40例。用酶联免疫分析定量检测各组人群血清GP73含量,微量离心柱分离AFPL3,罗氏电化学发光法测定总AFP和分离洗脱液中AFP-L3浓度,并计算AFP-L3占总AFP的比例(%)。结果:各组血清GP73和AFP-L3水平比较,差异均有统计学意义(P均<0.01),肝癌组阳性检出率均明显高于其他各组(P均<0.01)。GP73、AFP-L3和GP73+AFP-L3诊断小肝癌的灵敏度、特异性、准确率和曲线下面积分别为72.0%、93.3%、87.1%和0.820,62.0%、92.5%、83.5%和0.813,76.0%、96.7%、90.6%和0.870。结论:单独检测GP73和AFP-L3对低浓度AFP小肝癌的诊断价值较高,二者联合检测可提高诊断价值,用于AFP低浓度小肝癌的辅助诊断。Objective ：To investigate significance of combined test of serum golgi glycoprotein 73 （ GP73 ） and lens culinaris agglutinin - reactive alpha - fetoprotein （ AFP - L3 ） in diagnosis of small primary hepatocellular carcinoma （PHC） with negative alpha - fetoprotein （AFP）. Methods： A total of 50 cases of small PHC with negative AFP, 80 cases of non - PHC digestive system disorders and 40 cases of healthy controls were collected. Serum GP73 was ana- lyzed by enzyme- linked immunosorbent assay. AFP- L3 was separated by microspincolumn, the levels of AFP- L3 and total AFP were quantified by electrochemiluminescence immunoassays ,percentage of AFP -L3 to total AFP was calculated. Results：Levels of GP73 and AFP - L3 were all significantly different among three groups （P 〈 0.01 ）. Positive detection rates of two biomarkers in PHC were higher than other groups （ P 〈 0. O1 ）. The sensitivities, spe- cificities, accuracy rates and areas under ROC curve were 72.0% ,93.3% ,87.1% and 0. 820 by GP73,62.0% , 92.5% ,83.5% and 0. 813 by AFP - L3,76.0% ,96.7% ,90.6% and 0. 870 by GP73 ＋ AFP - L3. Conclusion： GP73 and AFP - L3 are superior biomarkers to aid the diagnosis of small PHC with negative AFP. The combined de- tection of biomarkers improved the diagnostic accuracy.

关 键 词：原发性肝癌 血清标志物 联合检测 诊断 

分 类 号：R735.7[医药卫生—肿瘤]