高通量测序在动物检疫中的应用进展  

Progress on application of high-throughput sequencing in animal quarantine

作　　者：孙涛[1] 李明哲[1] 崔淑华[1] 尹伟力 

机构地区：[1]山东出入境检验检疫局,青岛266002 [2]烟台出入境检验检疫局,烟台264000

出　　处：《食品安全质量检测学报》2017年第5期1693-1697,共5页Journal of Food Safety and Quality

基　　金：国家质量监督检验检疫总局基金项目(2015IK194)~~

摘　　要：面对无法预测、爆发突然及传播迅速的疫情,快速获知病原信息并真实地反映样品及环境中病原的多样性资料是各国卫生部门及口岸预警的核心工作之一。应用高通量测序技术全面监控研究复杂病原样品,是协助寻找传染溯源、防控动物疫病可行性的一条重要思路。目前,高通量测序在动物检疫上的应用与传统的病原研究方式最大的区别在于把病原微生物看成一个整体,摆脱了对单一病原分离培养的步骤,直接对样品基质中所有病原进行研究。随着测序技术和数据处理分析能力的飞速发展,以高通量测序为基础的宏基因组学研究已渗透到各个领域,在畜产品、养殖环境等样本检测方面显示了重要价值。本文对高通量测序的研究方法及在检验检疫上的应用趋势进行了综述。In the face of unpredictable,suddenly broken,rapidly spread epidemic,learning agent information quickly and reflecting the pathogens diversity of samples is one of the core work for the national health authorities and the port early warning.With the application of high-throughput sequencing technologies,monitoring complex samples comprehensively is a good idea to trace or prevent animal epidemic.At present,the biggest difference between the traditional way and the method of high-throughput sequencing in animal quarantine is that the pathogenic microorganisms is regarded as a whole,which gets rid of the cultivation of a single pathogen,and all pathogen in the matrix of the sample are directly studied.With the development of sequencing technology and the data processing analysis,metagenomics research has been applied in all fields and shows very important value,such as animal by-products,farming environment samples.This article briefly summarised the methods and application trends of high-throughput sequencing technologies in inspection and quarantine.

关 键 词：宏基因组学 高通量测序 动物检疫 

分 类 号：S851.34[农业科学—预防兽医学]