RNA干扰沉默IQGAP1表达可提高食管鳞癌细胞KYSE150对顺铂的化疗敏感度  

Sensitivity of Esophageal Squamous Carcinoma Cell Line KYSE150 to Cisplatin Can Be Improved by RNA-interfered Expression of IQGAP1

作　　者：崔发财[1] 陈瑜 秦望森[1] 胡敏[1] 魏晓霞[1] 

机构地区：[1]河南省人民医院检验科,郑州450003 [2]郑州大学附属肿瘤医院病理科,郑州450008

出　　处：《肿瘤防治研究》2017年第6期387-391,共5页Cancer Research on Prevention and Treatment

基　　金：河南省医学科技攻关计划项目(201303155)

摘　　要：目的研究具有IQ结构域的GTP激酶活化蛋白1基因(IQGAP1)对食管癌细胞KYSE150化疗敏感度的影响及其相关作用机制。方法构建针对IQGAP1基因的小干扰RNA(si RNA)并转染至食管鳞癌细胞株KYSE150,采用MTT法观察KYSE150细胞对化疗药物顺铂敏感度的改变;流式细胞技术检测IQGAP1基因沉默前后顺铂对KYSE150细胞周期分布及凋亡的影响;Western blot检测细胞转染前后转录因子E2相关因子2(nuclear factor erythroid-2 related factor 2,Nrf2)和多药耐药相关蛋白1(MRP1)的表达水平变化。结果转染si RNA可显著下调KYSE150细胞中的IQGAP1 m RNA和蛋白表达水平;经(1、5、10、20)μmol/L顺铂处理细胞24 h后,干扰组(IQGAP1-si RNA)的细胞生存率均较对照组发生明显下降(P<0.05);流式细胞仪检测结果显示干扰组在顺铂处理后G_0/G_1期细胞比例和凋亡率均明显高于对照组(P<0.05);Western blot结果显示IQGAP1基因沉默后,Nrf2和MRP1蛋白表达下调。结论沉默IQGAP1基因可有效提高人食管鳞癌细胞株KYSE150对顺铂的化疗敏感度,该作用可能是通过下调Nrf2和MRP-1蛋白表达来实现的。Objective To investigate the effects of RNA-interfered IQGAP1 gene on the sensitivity of esophageal squamous cell line KYSE150 to cisplatin（DDP） and the relevant mechanisms. Methods Synthesized siRNA targeting IQGAP1 was transfected into KYSE150 cells. MTT assay was employed to observe the sensitivity of KYSE150 cells to cisplatin. Flow cytometry assay was used to detect the cell cycle proportion and apoptosis rate in the interference group, blank control group and negative control group. The expression of Nrf2 and MRP-1 protein in KYSE150 cells were detected by Western blot. Results Transfection of siRNA could significantly inhibit the expression of IQGAP1 protein and mRNA in ESCC cell lines KYSE150. After cisplatin （1, 5, 10, 20） μmol/L treatment for 24 hours, cell survival rates in the interference groups were obviously decreased, compared with control groups（P〈0.05）; flow cytometry showed that cells proportion in G0/G1 phase and apoptotic rate in the interference group were obviously increased, compared with control groups（P〈0.05）; Western blot revealed that IQGAP1 gene silencing down- regulated the expression of Nrf2 and MRP-1 protein. Conclusion The sensitivity of esophageal squamous carcinoma cells to cisplatin can be improved by siRNA-interfered expression of IQGAP1, and this effect may be achieved by down-regulating Nrf2 and MRP-1 protein expression.

关 键 词：化疗敏感度 具有IQ结构域的GTP激酶活化蛋白1 食管鳞癌 小干扰RNA 转录因子E2相关因子2 

分 类 号：R735.1[医药卫生—肿瘤] R73-34[医药卫生—临床医学]