小干扰RNA沉默YKL-40基因对子宫内膜癌HEC-1A细胞顺铂化疗敏感度的影响  

Effects of si RNA Targeting YKL-40 Gene on Chemosensitivity of Endometrial Cancer HEC-1A Cells to Cisplatin

作　　者：李莉莉[1] 范江涛[1] 李大海[1] 刘炎[2] 

机构地区：[1]广西医科大学第一附属医院妇产科,南宁530021 [2]湖北省十堰市太和医院妇产科,湖北医药学院,十堰442000

出　　处：《肿瘤防治研究》2017年第6期398-402,共5页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金(81360388)

摘　　要：目的通过si RNA沉默YKL-40基因探讨其对子宫内膜癌HEC-1A细胞顺铂化疗敏感度的影响。方法 si RNA基因干扰技术沉默YKL-40基因,将人子宫内膜癌HEC-1A细胞分为三组:空白对照组、空载体组、si RNA实验组。q RT-PCR法检测沉默效率。q RT-PCR法检测顺铂处理子宫内膜癌细胞对癌细胞中YKL-40基因的影响。MTT法和流式细胞术检测靶细胞的增殖能力以及凋亡率。结果转染组YKL-40m RNA的表达量显著低于空白对照组和空载体组(P=0.036,P=0.005),经过相同浓度的顺铂处理子宫内膜癌后,YKL-40基因的表达量上调。转染组的增殖能力低于空白对照组和空载体组(P<0.05),而凋亡率高于空白对照组和空载体组(P=0.000,P=0.000)。结论 YKL-40基因沉默可以提高子宫内膜癌HCE-1A细胞对顺铂的敏感度。YKL-40基因具有抗凋亡作用。Objective To explore the effects of silencing YKL-40 gene by small interfering RNA （siRNA） on the chemosensitivity of endometrial cancer HEC-1A cells to cisplatin. Methods We silenced the YKL-40 gene in HEC-1A cells using the siRNA technique and divided the HEC-1A cells into three groups as blank control group, mock-treatment group and experimental group, respectively. The mRNA expression of YICL-40 was measured using quantitative real time-PCR（qRT-PCR） assay after treated with cisplatin. The proliferation and apoptosis of HEC-1A cells were also determined using MTT assay and flow cytometry（FCM） respectively. Results The mRNA expression of YKL-40 in the experimental group was significantly lower than those in blank control and mock-treatment groups（P=0.036, 0.005, respectively）. The mRNA expression of YKL-40 in HEC-1A cells was up-regulated after treatment of HEC-1A cells with the same concentration of cisplatin. The proliferative ability of HEC-1A cells was decreased（P〈0.05） while the apoptosis was increased（P=0.000） significantly in the experimental group, compared with the other two groups. Conclusion The chemosensitivity of HCE-1A cells to cisplatin could be elevated by silencing YKL-40 gene. YKL-40 gene in HCE-1A cells have the ability of anti-apoptosis.

关 键 词：YKL-40 SIRNA 子宫内膜癌 顺铂 化疗 

分 类 号：R737.33[医药卫生—肿瘤]