松萝抗癌活性部位对人泌尿生殖系统肿瘤的抑制作用  

Anticancer Effects of Usnea Diffracta Vain Bioactive Fraction on Human Urogenital Cancers

作　　者：王静[1] 贺小琼[2] 姚乾[3] 李魁梧 胡松杉 贺云发 

机构地区：[1]西安医学院第二附属医院体检中心,西安710038 [2]昆明医科大学公共卫生学院,昆明650500 [3]昆明医科大学第三附属医院,云南省肿瘤研究所,昆明650031 [4]解放军第四七八医院骨科,昆明650031

出　　处：《肿瘤防治研究》2017年第6期403-408,共6页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金(30960437);云南省科技计划项目(2009FXL001);云南省科技厅-昆明医科大学联合专项重点项目(2012FB001);昆明医科大学重大培育项目(CGPY-201603)

摘　　要：目的研究松萝提取物AMH-T对人泌尿生殖系统肿瘤细胞的体内、体外抗癌作用,观察AMH-T与DDP的体外联合抗癌作用以及对动物的一般毒性,探讨其诱导癌细胞凋亡的可能机制。方法 MTT法检测体外抗癌作用,裸鼠移植瘤试验进行体内抗He La肿瘤研究,测量法观察AMH-T对小鼠体重和脏器发育的影响。荧光法检测癌细胞凋亡与Caspase-8信号通路的关系。结果随着AMH-T浓度增加和作用时间的延长,ACHN、T-24、PC-3、He La等癌细胞死亡增加。AMH-T浓度为8μg/ml时,72 h后癌细胞绝大部分死亡。与DDP联合用药对各癌细胞株均具有体外协同抗肿瘤作用。AMH-T剂量50 mg/kg时,He La移植瘤的相对增殖率为23.67%,肿瘤重量抑制率达到54.20%。AMH-T能影响小鼠肝脏发育,对脾脏、肾脏、心脏和睾丸发育没有显著性影响。AMH-T不影响Caspase-8活性。结论 AMH-T对人泌尿生殖系统肿瘤具有显著性体内和体外抗癌作用,与DDP联合用药具有体外协同抗肿瘤作用;AMH-T诱导癌细胞凋亡与Caspase-8信号通路无关。Objective To investigate the in vitro and in vivo anticancer effects of Usnea Diffracta Vain bioactive fraction AMH-T on human urogenital cancers, to observe the in vitro combination anticancer effects of AMH-T with cisplatin （DDP） and the physical toxicity of AMH-T, and to discuss the related mechanisms. Methods MTT assay was used to measure the in vitro anticancer activity. The in vivo anticancer effects on HeLa tumor were tested in xenograft cancer model in balb/c nude mice. Mice body weight and organ coefficients were measured. Caspase-8 activity was detected by kit. Results Cancer cells, ACHN, PC-3, T-24 and HeLa, died increasingly with the increasing AMH-T concentration and time. Cancer cells almost completely died at 8~tg/ml of AMH-T after 72h. AMH-T and DDP showed synergistic in vitro anticancer effect on cancer cells. Treated with 50mg/kg of AMH-T, the relative proliferation ratio of HeLa tumor was 23.67% and the weight inhibitory ratio was 54.20%. No significant difference was found in organ coefficients of spleen, kidney, heart and testis except liver. Caspase-8 activity was not affected by AMH-T. Conclusion AMH-T has significant in vitro and in vivo anticancer effects on human urogenital cancers. AMH-T and DDP have synergistic in vitro anticancer effects. The apoptosis induced by AMH-T has no relationship with Caspase-8 signaling pathway.

关 键 词：松萝提取物AMH-T DDP ACHN PC-3 T-24 HELA 

分 类 号：R737.1[医药卫生—肿瘤] R730.52[医药卫生—临床医学]