超高效液相色谱-串联质谱法同时检测植物叶片中吲哚-3-乙酸及其3种氧化产物  

Simultaneous Determination of Indole-3-acetic Acid and Its Three Oxidative Products in Plant Leaves by UPLC-MS/MS

作　　者：刘志航[1] 李平亮[2] 周斐[2] 罗小勇[2] 

机构地区：[1]青岛农业大学化学与药学院,山东青岛266109 [2]青岛农业大学农学与植物保护学院,山东青岛266109

出　　处：《分析测试学报》2017年第6期732-737,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(31272066)

摘　　要：以玉米叶片为供试材料,建立了同时测定植物体内吲哚-3-乙酸(IAA)及其3种氧化产物吲哚-3-甲醇(ICI)、吲哚-3-甲醛(ICA)、吲哚-3-羧酸(IFA)含量的超高效液相色谱-串联质谱(UPLC-MS/MS)方法。结果表明,该方法对IAA及其3种氧化产物检测的线性、精密度和重复性较好,灵敏度较高,4种化合物的检出限为0.002~1.63μg/kg,定量下限为0.007~5.43μg/kg;方法的加标回收率为89.5%~95.3%,相对标准偏差为2.3%~5.1%。玉米叶片的实际测定结果表明,IAA,ICI,ICA和IFA的含量分别为(196.25±7.10),(26.21±2.13),(18.65±2.02),(13.62±2.06)μg/kg。该方法已成功应用于小麦、豌豆、硬毛刺苞菊叶片的测定,通用性较好。An ultra-performance liquid chromatography-tandem mass spectrometric（UPLC-MS/MS） method was developed for the simultaneous determination of indole-3-acetic acid（IAA） and its three oxidative products, indole-3-methanol（ICI）,indole-3-carboxaldehyde（ICA） and indole-3-carboxylic acid（IFA） in corn（Zea mays L.） leaves. The results showed that the detection limits for the four compounds were in the range of 0.002-1.63μg/kg, and the quantitation limits were 0.007-5.43μg/kg. The recoveries ranged from 89.5% to 95.3% with RSDs of 2.3%-5.1%. The method was applied in the determination of the contents of IAA,ICI,ICA and IFA in corn leaves with their results of （196.25±7.10）,（26.21±2.13）,（18.65±2.02）,（13.62±2.06）μg/kg, respectively. Meanwhile, the proposed method was also successfully applied in the analysis of wheat（Triticum aestivum L.）,pea（Pisum sativum L.） and bristly starbur（Acanthospermum hispidum DC.）,which indicated that the method had a good generality.

关 键 词：玉米叶片 吲哚-3-乙酸 吲哚-3-甲醇 吲哚-3-甲醛 吲哚-3-羧酸 超高效液相色谱-串联质谱(UPLC-MS/MS) 

分 类 号：O657.63[理学—分析化学]