膀胱尿路上皮癌中PTEN表达与其基因甲基化的关系  

Correlation between expression of PTEN and its promoter methylation in bladder urothelial cell carcinoma

作　　者：尹永华[1] 程文杰[2] 延敏博[2] 

机构地区：[1]广州医科大学附属深圳沙井医院泌尿外科,广东深圳518104 [2]中山大学附属第五医院泌尿外科,广东珠海519000

出　　处：《中国现代医学杂志》2017年第12期46-49,共4页China Journal of Modern Medicine

基　　金：广东省深圳市科技计划项目(No:JCYJ20140414160300578)

摘　　要：目的探讨膀胱尿路上皮癌(BUCC)中第10号染色体同源丢失性磷酸酶-张力蛋白基因(PTEN)表达与其基因启动子甲基化的关系。方法应用Western blot、甲基化特异性聚合酶链反应(MSP),检测41例BUCC和18例正常膀胱组织中PTEN蛋白的表达水平及其基因启动子甲基化状态,并分析两者的相关性。结果BUCC中PTEN蛋白表达低于正常膀胱组织(P<0.05),而BUCC中PTEN基因启动子甲基化率为53.66%(22/41),高于正常膀胱组织的0.00%(0/18)(P<0.05);与甲基化阴性组的BUCC组织相比,22例甲基化阳性组的PTEN表达水平降低(P<0.05)。结论 BUCC中PTEN蛋白的表达水平下调,这与其基因启动子高甲基化密切相关。Objective To investigate the relationship between expression of phosphate and tension homology deleted on chromsome ten （PTEN） gene and its promoter methylation in bladder urothelial cell carcinoma （BUCC）. Methods The protein expression and promoter methylation of PTEN gene were detected in 41 cases of BUCC and 18 normal bladder tissues using Western blot and Methylation Specific PCR （MSP） method respectively, and their correlation was analyzed. Results PTEN protein expression in the BUCC was lower than that in the normal bladder tissues （P 〈 0.05）. And the promoter methylation rate of PTEN gene in the BUCC （53.66%, 22/41） was significantly higher than that （0.00%, 0/18） in the normal bladder tissues （P〈 0.05）. The expression level of PTEN protein in the BUCC of the methylation negative group was obviously lower than that of the methylation positive group （P 〈 0.05）. Conclusions The low expression of PTEN protein in BUCC is closely related with the high methylation of PTEN gene promoter.

关 键 词：膀胱尿路上皮癌 磷酸酶-张力蛋白基因 DNA甲基化 

分 类 号：R737.14[医药卫生—肿瘤]