慢性束缚应激联合番泻叶灌胃法制备IBS-D大鼠模型的量效及时效关系评价  

Dose-effect and time-effect relationship of chronic restraint stress combined with senna extract gavage in inducing diarrhea-predominant irritable bowel syndrome in rats

作　　者：赵妍[1] 罗丹妮[1] 陈颖[1] 黄辰[1] 周思远[1] 

机构地区：[1]成都中医药大学针灸推拿学院,四川省成都市610075

出　　处：《世界华人消化杂志》2017年第15期1360-1367,共8页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81503665~~

摘　　要：目的观察不同番泻叶浓度、束缚时间及造模持续时间对腹泻型肠易激综合征(diarrhea predominant irritable bowel syndrome,IBS-D)大鼠模型造成的影响,筛选一种较为理想的IBS-D大鼠模型制作方法.方法 SD大鼠32只,雌雄各半.随机分为空白组、模型1组(0.2 g/mL番泻叶+束缚1 h)、模型2组(0.3 g/mL番泻叶+束缚1 h)、模型3组(0.2 g/mL番泻叶+束缚1.5 h),每组8只,雌雄各半.以造模7、10、14 d时腹壁撤退反射时的疼痛阈值评价其内脏敏感性,腹泻指数评价其腹泻程度,同时对肠黏膜的组织学改变进行评价.结果各组模型大鼠疼痛阈值在7、10、14 d均较空白组明显降低(P<0.05);腹泻指数在7、10、14 d均较空白组明显增加(P<0.05).模型2组的疼痛阈值较模型1组在7 d和14 d低(均为P=0.006),与模型3组比较差异不明显(P=1.000,P=0.198);模型2组的腹泻指数较模型1组和模型3组在3个时间点高(P<0.05).组织学分析显示各组大鼠均无明显的炎症性表现.结论 3种造模方法在7、10、14 d均可建立IBS-D的动物模型,其中束缚1 h联合番泻叶0.3 g/mL灌胃的方法在内脏敏感性及腹泻指数上要优于其他两种造模方法.AIM To observe the effects of gavage with different concentrations of senna for different durations plus different durations of chronic restraint stress （CRS） in inducing diarrhea-predominant irritable bowel syndrome （IBS-D） in rats, in order to optimize the creation of the rat model of IBS-D. METHODS Thirty-two SD rats were randomly and equally divided into four groups： A （0.2 g/mL senna ＋ CRS for 1 h）, B （0.3 g/mL senna ＋ CRS for 1 h）, C （0. 2 g/mL senna ＋ CRS for 1.5 h）, and D （normal control group）. The pain threshold of abdominal wall withdrawal reflex was evaluated at 7, 10, and 14 d. Diarrhea index was used for evaluation of the degree of diarrhea, and histological changes in the intestinal mucosa were also evaluated. RESULTS The pain threshold of each model group was significantly lower than that of the normal control group at 7, 10, and 14 d （P 〈 0.05）. Diarrhea indexes at 7 d, 10 d and 14 d were significantly increased in groups A-C compared with group D （P 〈 0.05）. Pain threshold at 7 and14 d in group B was significantly lower than that in group A （P = 0.006 for both）, but did not differ significantly when compared with group C （P = 1.000, P = 0.198）. Diarrhea indexes at all three time points were significantly higher in group B than in groups A and C （P 〈 0.05）. Histological analysis showed that the rats in each group had no significant inflammatory manifestations. CONCLUSION CRS for 1 h plus 0.3 g/mL senna is superior to other parameters in inducing IBS-D in rats with regard to visceral sensitivity and diarrhea index.

关 键 词：慢性束缚应激 番泻叶浸剂灌胃 IBS-D大鼠模型 量效及时效关系 

分 类 号：R-332[医药卫生] R574.4