密骨方对去卵巢大鼠骨折免疫炎症因子的影响  

Effect of Migufang on Immune Inflammatory Cytokines in Fractures of Ovariectomized Rats

作　　者：周丹丹[1] 张梓瑞 李强[3] 杜原瑗[4] 汪学红[1] 

机构地区：[1]武汉体育学院,武汉430079 [2]四川大学华西医学院 [3]十堰市太和医院 [4]湖北省肿瘤医院

出　　处：《中国中医骨伤科杂志》2017年第6期6-9,14,共5页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：湖北省自然科学基金资助项目(2011CDB312);武汉体育学院青年教师科研基金资助项目(2014QZ09)

摘　　要：目的:切除Wistar大鼠卵巢后致其股骨骨折,行内固定术建立动物模型,探讨密骨方对骨质疏松后骨折免疫炎症因子的影响。方法:将45只雌性Wistar大鼠随机分为假手术对照组(Sham)、骨质疏松骨折模型组(OVX)、骨质疏松骨折密骨方组(MGF)。模型组及密骨方组行卵巢摘除术,并在5个月后骨折造模。密骨方组按100mg/(kg·d)剂量的密骨方汤剂灌胃给药,对照组和模型组给予同体积的生理盐水,1次/d,分别在第14,28,42天各取5只断头取材,ELISA法测定血清TNF-α和Tracp的含量,SABC法检测骨折端IL-6的表达。结果:OVX组TNF-α,IL-6及Tracp在时间的变化上呈显著升高趋势,差异有统计学意义(P<0.05);密骨方组中TNF-α,IL-6及Tracp在时间的变化上呈显著下降趋势,差异有统计学意义(P<0.05)。结论:中药密骨方能显著降低大鼠血清中炎症因子TNF-α的含量和骨折端炎症因子IL-6的表达,诱导Tracp在血清中的含量下降,促进骨质疏松后骨折愈合。Objective： To establish the animal model of ovariectomy induced femoral fractures for internal fixation in the Wistar rats, and to explore the effect of Migufang on immune inflammatory cytokines in osteoporotic fractures. Methods： All 45 female Wistar rats were randomly divided into the sham operation group（sham）, the osteoporotic fractures model group（OVX）and the osteoporotic fractures Migufang group（MGF）. The rats of OVX group and MGF group were ovariectomized and the fractures models were established after 5 months. The rats of MGF group were given an intragastrical dose of 100 mg/（kg·d）Migufang, and the sham group and OVX group were given the same volume of saline, once a day. Five rats from each group were decapitated to take material at 14 th, 28 th, and 42 th days, respectively. The levels of TNF-α and Tracp in serum were measured by ELISA methods, and the expression of IL-6 in the fractures was detected by SABC method. Results： The levels of TNF-α, IL-6, and Tracp in OVX group were significantly increased in the change of time（P〈0.05）, while the levels of TNF-α, IL-6, and Tracp in MFG group were significantly decreased in the change of time（P〈0.05）. Conclusion： MGF can significantly reduce the content of inflammatory cytokines TNF-α in the serum and the expression of inflammatory factor IL-6 in the fractures of rats, and induce the decrease of the content of Tracp in the serum to promote osteoporotic fracture healing.

关 键 词：密骨方 骨质疏松后骨折 免疫炎症因子 

分 类 号：R-33[医药卫生]