广西肝癌高发区原发性肝细胞癌HBV X基因的整合及影响因素分析  

Analysis of hepatitis B virus X gene integration and potential risk factors of integration in patients with hepatocellular carcinoma in the high-incidence area of Guangxi

作　　者：吴杭航 黄天壬[2] 邓伟[1] 方向[1,2] 任静静[1,2] 甘膺元 

机构地区：[1]广西医科大学附属肿瘤医院实验研究部,南宁530021 [2]广西医科大学研究生院

出　　处：《中国癌症防治杂志》2017年第3期172-176,共5页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：国家自然科学基金资助项目(81660561;81260319);广西高校科学技术研究资助项目(KY2015ZD025;ZD2014039)

摘　　要：目的了解广西肝癌高发区乙型肝炎病毒(hepatitis B virus,HBV)X区基因在肝细胞癌(hepatocellular carcinoma,HCC)染色体中的整合及影响因素。方法以30例与HBV相关的原发性肝细胞癌患者为研究对象。提取HCC组织及癌旁组织标本DNA作为模板,以HBV X基因上游序列和人类基因组Alu重复序列为引物,应用重复序列-多聚酶链反应(Alu-PCR)扩增整合的HBV X片段及两侧的人类基因组DNA片段。扩增产物进行测序,计算目的片段整合率并分析相关的影响因素。结果 18例HCC组织检测到HBV X基因的整合片段,整合率为60.00%(18/30);26例癌旁组织检测到HBV X基因的整合片段,整合率为86.67%(26/30)。癌旁组织HBV病毒整合率高于HCC组织,差异有统计学意义(χ~2=5.445,P=0.020)。不同性别、年龄、HBe Ag、HBV DNA、ALT、AST的HCC癌组织及其癌旁组织HBV X基因整合率比较差异均无统计学意义(P>0.05)。结论广西肝癌高发区癌旁组织比HCC组织HBV整合率高,说明HBV整合发生在感染早期。HBV X基因整合与HCC患者性别、年龄、HBe Ag、HBV DNA、ALT、AST无明显关系。Objective To analyze integration of hepatitis B virus （HBV） X gene into the genome of primary hepatocellular carcinoma （HCC） tumors and surrounding tissue, and to explore potential risk factors of such integration in patients from the HCC high-incidence area of Guangxi. Methods DNA was extracted from HCC tissue and adjacent non-tumor liver tissue of 30 patients with HBV-related HCC. Primers were designed to amplify the HBV X sequence and human Alu repeats by Alu-PCR,and PCR products were Sanger- sequenced. Results Among the 30 patients, 18 （60.00%） showed integration of the HBV X gene into HCC tumors,compared to 26 （86.67%） who showed X gene integration into adjacent liver tissue （Х^2=5.445,P=0.020）. Incidence of X gene integration did not vary significantly with sex, age, HbeAg status, HBV DNA load, ALT or AST in HCC tissue or adjacent non-tumorous liver tissue （P〉0.05）. Conclusions Incidence of HBV integration is higher in non-tumorous tissues than in tumorous tissues in patients with HBV-related HCC in Guangxi.HBV integration appears to occur in early stages of HCC development. HBV X gene integration does not appear to be influenced by sex, age, HBeAg status, HBV DNA load, ALT or AST.

关 键 词：肝肿瘤 乙型肝炎病毒X基因 整合 广西 

分 类 号：R735.7[医药卫生—肿瘤]