Electromyography and Fos immunostaining study establish a possible functional link between trigeminal proprioception and the oculomotor system in rats  

作　　者：Houcheng Liang Jingdong Zhang Pifu Luo Hongna Zhu Ying Qiao Anle Su Ting Zhang 

机构地区：[1]Xi'an BRIGHT Eye Hospital,Xi'an,Shaanxi 710000,China [2]Shaanxi Provincial Eye Research Institute and Xi'an Eye Hospital Xi'an,Shaanxi 710002,China [3]Department of Ophthalmology,2nd Affiliated Hospital of Xi'an Jiao-Tong University,Xi'an,Shaanxi 710002,China [4]Department of Pathology,KingMed Diagnostics,lnternational Bioisland,Guangzhou,Guangdong 510050,China

出　　处：《The Journal of Biomedical Research》2017年第3期256-263,共8页生物医学研究杂志（英文版）

基　　金：partially supported by Natural Sciences Research Funding 2006C225 and 2009K0174 from Shaanxi Province awarded to Dr.Liang HC

摘　　要：The objective of this study was to explore whether there was a functional link between trigeminal proprioception and the oculomotor system mediated through jaw muscle afferents.Electromyography（EMG） was undertaken of the levator palpebrae（LP） and superior rectus（SR）,and Fos expression was detected in the brainstem following consecutive down-stretching of the lower jaw at 2-4 Hz in rats.Retrograde tracing was undertaken of the interstitial nucleus of Cajal and Darkschewitsch nucleus（INC/DN） pre-oculomotor neurons.FMG-like responses were recorded from the LP/SR during down-stretching of the lower jaw at 2-4 Hz in 3 out of 11 rats.Fos expression was induced by consecutive down-stretching of the lower jaw at 2-4 Hz for 20-30 seconds.Interestingly,Fos expression was distributed mainly in the bilateral INC/DN area.We also examined Fos-like immunoreactivity in central mesencephalic and paramedian pontine reticular formation that harbors premotor neurons controlling horizontal eye movement,but no Fos-like staining was observed therein.By injection of retrograde tracers into the oculomotor nucleus combined with Fos immunostaining,double labeled pre-oculomotor neurons were visualized to distribute in the INC/DN.In conclusions,there may exist a trigeminal proprioceptive-oculomotor system neural circuit through jaw muscle afferents in rats.Judging from Fos distribution pattern,this pathway might be related to vertical and torsional eye movements.The objective of this study was to explore whether there was a functional link between trigeminal proprioception and the oculomotor system mediated through jaw muscle afferents.Electromyography（EMG） was undertaken of the levator palpebrae（LP） and superior rectus（SR）,and Fos expression was detected in the brainstem following consecutive down-stretching of the lower jaw at 2-4 Hz in rats.Retrograde tracing was undertaken of the interstitial nucleus of Cajal and Darkschewitsch nucleus（INC/DN） pre-oculomotor neurons.FMG-like responses were recorded from the LP/SR during down-stretching of the lower jaw at 2-4 Hz in 3 out of 11 rats.Fos expression was induced by consecutive down-stretching of the lower jaw at 2-4 Hz for 20-30 seconds.Interestingly,Fos expression was distributed mainly in the bilateral INC/DN area.We also examined Fos-like immunoreactivity in central mesencephalic and paramedian pontine reticular formation that harbors premotor neurons controlling horizontal eye movement,but no Fos-like staining was observed therein.By injection of retrograde tracers into the oculomotor nucleus combined with Fos immunostaining,double labeled pre-oculomotor neurons were visualized to distribute in the INC/DN.In conclusions,there may exist a trigeminal proprioceptive-oculomotor system neural circuit through jaw muscle afferents in rats.Judging from Fos distribution pattern,this pathway might be related to vertical and torsional eye movements.

关 键 词：Marcus Gunn syndrome down-stretching lower jaw levator and superior rectus electromyography interstitial nucleus of Cajal and Darkschewitsch nucleus Fos expression 

分 类 号：R77[医药卫生—眼科]