过表达STAT1与CD74CD44促进结肠癌细胞粘附和迁移的相关性  

Overexpression of STAT1 and CD74CD44 to Promote Adhesion and Migration of Colon Cancer Cells

作　　者：徐瑞敏[1,2] 陈志康[1] 甘惠玲 陈泽伦 

机构地区：[1]中南大学湘雅医院,410008 [2]海南省农垦总医院,570000

出　　处：《实用癌症杂志》2017年第7期1094-1098,共5页The Practical Journal of Cancer

摘　　要：目的探讨信号转导和转录激活子1(STAT1)与CD74/CD44在结肠癌中异常表达与肿瘤转移的相关性。方法培养结肠癌细胞株Ls-174-T,待其生长至对数生长期,传至第3代收集肿瘤细胞,各细胞分为3份。1份行RTPCR、Western blotting方法检测STAT1与CD74/CD44表达情况;1份检测定量粘附细胞数;1份以划痕实验检测细胞迁移能力。统计分析比较STAT1与CD74/CD44在结肠癌细胞中的异常表达情况及与肿瘤迁移和粘附能力的相关性。结果RT-PCR、Western blotting结肠癌组织中STAT1与CD74/CD44的蛋白及mRNA表达显著高于正常结肠组织,两组相比具有统计学差异(P<0.05)。MTT法检测结果显示,不同作用时间(0、6、12、24、48 h)不同剂量STAT1与CD74/CD44过表达质粒(0.000、0.001、0.010、0.060、0.100 mg/ml)干预后,结肠癌细胞体外粘附能力明显加强,与对照组(0.000 mg/ml各组)相比,差异有统计学意义;Transwell结果显示,不同作用时间(0、6、12、24、48 h)不同剂量STAT1与CD74/CD44过表达质粒(0.000、0.001、0.010、0.060、0.100 mg/ml)干预后,结肠癌细胞体外迁移能力随着时间及剂量的增加而明显加强,与对照组(0.000mg/ml各组)相比,差异有统计学意义。结论 STAT1与CD74/CD44在结肠癌细胞中高表达,从而调节结肠癌细胞粘附和迁移能力的作用,表明STAT1与CD74/CD44在结肠癌发生进展中可能与肿瘤的侵袭和转移密切相关。Objective To investigate the signal transducer and activator of transcription 1 （STAT1） and CD74 / CD44 expression in colon cancer cells with abnormal tumor metastasis. Methods Cultured colon cancer cell line Ls-174-T,let it grow to a logarithmic growth phase, the third passage collected tumor ceils, each cell was divided into 3 parts. A line of RT-PCR, West-ern blotting method was used to detect STAT1 and CD74/CD44 expression ; a quantitative detection of adherent cells ; a scratch test to detect cell migration. Statistical analysis and comparison of STAT1 and CD74 / CD44 in colon cancer ceils and abnormal expression correlated with tumor migration and adhesion ability. Results The expression of STAT1 and CD74 / CD44 protein in colorectal cancer tissues was significantly higher than that in normal colorectal tissues （P 〈 0.05 ）. MTr assay test results showed that different time （0,6,12,24,48 h） and different doses of STAT1 and CD74/CD44 over-expression plasmid （0. 000,0. 001, 0. 010,0.060,0. 100 mg/ml） intervention colon cancer cells In vitro adhesion significantly strengthened with the control group （0.000 mg/ml in each group）, the difference was statistically significant ;Transwell results showed that different time （0,6,12, 24,48 h） and different doses of STAT1 CD74/CD44 over-expression plasmid （0.000,0. 001,0. 010,0.060,0. 100 mg/ml） after the intervention of colon cancer cells in vitro migration with increasing dose and time and significantly strengthened with the con- trol group （0. 000 mg/ml in each group）, the difference was statistically significant. Conclusion STAT1 and CD74/CD44 is highly expressed in colon cancer cells, thereby regulating the role of colon cancer cell adhesion and migration, indicating that STAT1 and CD74 / CD44 may be associated with tumor invasion and metastasis in colon cancer progression.

关 键 词：转录激活子1(STAT1) CD74/CD44 结肠癌细胞 细胞粘附 迁移 

分 类 号：R735.35[医药卫生—肿瘤]