高F值玉米寡肽-锌螯合物的纯化及结构解析  

Purification and Structure Analysis of Zinc Chelate with High F Value of Corn Oligopeptide

作　　者：燕会 孙敏[2] 查世银 王元秀[2] 徐同成[3] 

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,山东济南250062 [2]济南大学生物科学与技术学院,山东济南250022 [3]山东省农业科学院农产品研究所,山东济南250100

出　　处：《泰山学院学报》2017年第3期102-108,共7页Journal of Taishan University

基　　金：国家高技术研究发展计划(863计划)(2013AA102206);山东省自然科学基金项目(SZR1527)

摘　　要：研究高F值玉米寡肽-锌螯合物的分离纯化及结构.利用膜分离系统、葡聚糖凝胶G-15和C-18反相高效液相对高F值玉米寡肽-锌螯合物进行分离纯化,运用紫外吸收光谱、电喷雾电离质谱争红外吸收光谱研究螯合物的结构.紫外全波长扫描结果显示,硫酸锌的最大吸收波长在192nm,高F值玉米寡肤的最大吸收波长在220nm,高F值玉米寡肽-锌螯合物的最大吸收波长在209nm;经过分离纯化高F值玉米寡肽-锌螯合物,得到组分P-Zn3a和P-Zn3b;采用MALDI-TOE/TOF MS/MS对组分进行测序,氨基酸序列为甘氨酸-缬氨酸-亮氨酸(异亮氨酸)(Gly-Val-Leu(Ⅱe))和甲硫氨酸-亮氨酸(异亮氨酸)(MetLeu(Ⅱe));红外光谱结构表征显示玉米寡肽与锌离子螯合机制为:与Zn^+发生螯合的位置可能是-NH-C=O上的-NH,-COOH上的-OH和末端-NH2.由此可初步推断,高F值玉米寡肽与锌发生了螯合并产生了一种新型的螯合物.Objective：To study the purification and structure of zinc chelate with high F value of corn oli- gopeptide. Methods： The zinc chelate with high F value of corn oligopeptide was isolated and purified by Mem- brane Separation System, Sephadex G - 15 and C - 18 reversed - phase high performance liquid chromatogra- phy. The structure of the chelate was studied by UV absorption spectroscopy, infrared absorption spectroscopy and electrospray ionization mass spectrometry. Results：Uv wavelength scanning all the results show that the maximum absorption wavelength of zinc sulfate in 192 nm, the maximum absorption wavelength of corn oli- gopeptides in 220 nm, the maximum absorption wavelength of peptide zinc chelate at 209 nm. The components P -Zn3a and P -Zn3b are acquired. The component are sequenced with MALDI -TOF/TOF MS/MS, and the amino acid sequence are Gly -Val -Leu（Ile） and Met -Leu（Ile）. The infrared spectrum of the structure indicated that the chelating mechanism of corn oligopeptide and zinc ion was as follows： the position of chela- tion with Zn2 ＋ may be - NH on - NH - C = O of oligopeptide, while - OH on - COOH and terminal - NH2 of oligopeptide. Conclusion ： It can be inferred that high F value of tom oligopeptides chela merger with zinc produced a new type of chelate.

关 键 词：高F值玉米寡肽 锌 螯合物 纯化 结构 

分 类 号：TS209[轻工技术与工程—食品科学]