基于特征荧光信号的去囊衣带芯橘瓣分选  

Sorting of peeled mandarin segments with orange core based on characteristic fluorescent signal

作　　者：王叶群[1] 杨增玲[1] 任卫东 刘婷[1] 杨杰[1] 张绍英[1] 

机构地区：[1]中国农业大学工学院,北京100083 [2]汇川盛业技术(北京)有限公司,北京100176

出　　处：《农业工程学报》2017年第9期296-301,共6页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家重点研发计划(2016YFD0400305)

摘　　要：为实现去囊衣带芯橘瓣的机器视觉分选,给去囊衣带芯橘瓣的机器识别提供直接、准确的判别信号,采用荧光分光光度计对50颗去囊衣带芯橘瓣的橘芯和砂囊分别进行三维荧光光谱扫描,通过对橘芯、砂囊平均三维荧光光谱分析,确定了橘芯相对砂囊的特征荧光信号,据此对带芯橘瓣荧光图像识别的可行性进行了验证。检测发现,在370~390 nm紫外光激发下,橘芯和砂囊在440 nm处的荧光强度差异较大;橘芯与砂囊在370 nm紫外光激发下,440 nm处荧光强度分布的箱线图表明两者荧光强度分布存在明显差异,且以橘芯在440 nm处荧光强度的下四分位数(Q1)与砂囊在440 nm处荧光强度的上四分位数(Q3)的平均值作为分类标准,去囊衣带芯橘瓣检出准确率可达85%。对(370±2)nm激发下得到的单色(440±5)nm荧光图像进行二值化及形态学处理后,可在以砂囊为背景的橘瓣图形中显现橘芯亮斑。利用橘芯与砂囊的荧光特性差异进行机器视觉成像分析,可作为识别去囊衣带芯橘瓣的一种有效方法。The peeled mandarin segment with orange core （central column residue） is one kind of defective mandarin segment during the production of canned mandarin. So it is necessary to remove the defective mandarin segments according to the requirement of quality before canning. Due to the differences of physical and chemical properties between orange core and orange gizzard, the fluorescence spectra of orange core and orange gizzard are different theoretically. For the purpose of offering direct and accurate signals to the machine to identify the peeled mandarin segments with orange cores, in this study, the peeled mandarin segments were taken as the samples, and the 3D （three-dimensional） fluorescence spectra of orange core and orange gizzard were determined by Cary Eclipse fluorescent speetrophotometer （excitation wavelength in the range of 250-700 nm, 5 nm interval; emission wavelength in the range of 300-800 nm, I nm interval; discharge voltage of 600 V, scanning speed of 1 200 nm/min）. The differences of fluorescence characteristics of orange core and orange gizzard were found according to the 3D fluorescence spectra after removing Rayleigh and Raman scatter. In order to verify the differences further, the fluorescence spectra of orange core and orange gizzard of 50 peeled mandarin segments were determined by Cary Eclipse fluorescent spectrophotometer （at an excitation wavelength of 370 rim, an emission wavelength in the range of 400-700 nm with the interval of 1 nm, a discharge voltage of 600 V, a scanning speed of 1 200 nm/min）. The differences of fluorescence intensities at 440 nm for orange core and orange gizzard were evaluated by means of drawing the box-plots of the fluorescence intensity at 440 nm. The accuracy rate of the discrimination of orange core and orange gizzard was analyzed, and the average value between fluorescence intensity of orange core at the lower quartile and that of orange gizzard at the upper quartile at 440 nm was used as classification criterion. The camera obscura was t

关 键 词：图像处理 光谱 荧光 去囊衣橘瓣 橘芯 砂囊 分选 

分 类 号：TP391.44[自动化与计算机技术—计算机应用技术]