冬凌草甲素影响细胞外排功能增加HONE1细胞对米托蒽醌的敏感性  

Oridonin increased the sensitivity of HONE1 to mitoxantrone by inhibiting the efflux function

作　　者：潘俊成[1] 邓嘉茵 胡杨[1] 刘丹阳[1] 王妍[3] 黄奕俊 

机构地区：[1]中山大学中山医学院,广东广州510080 [2]中山大学生命科学学院,广东广州510006 [3]中山大学附属第一医院,广东广州510080

出　　处：《热带医学杂志》2017年第5期557-559,587,F0003,共5页Journal of Tropical Medicine

基　　金：国家自然科学基金(81200095)

摘　　要：目的观察非毒性剂量冬凌草甲素是否增加鼻咽癌细胞株HONE1对米托蒽醌的敏感性。方法采用MTT法使用不同浓度的冬凌草甲素处理HONE1细胞株,确定冬凌草甲素的非毒剂量区间;然后用非毒性剂量冬凌草处理细胞后用流式细胞术进行侧群表型检测,探索能显著降低侧群表型的冬凌草甲素浓度;参考冬凌草甲素非毒性剂量和能降低侧群表型的剂量得到冬凌草甲素用于联用米托蒽醌的浓度。结果 5μmol/L冬凌草甲素并未对HONE1细胞产生明显毒性,该浓度处理细胞24 h后,细胞侧群比例由3.32%降低至0.55%;米托蒽醌联用5μmol/L冬凌草甲素组HONE1细胞存活率较单用米托蒽醌组低(P<0.05);流式细胞术分析显示膜外排蛋白ABCG2含量降低。结论冬凌草甲素可以通过影响细胞ABCG2外排功能增加鼻咽癌细胞株HONE1对米托蒽醌的敏感性。Objective To study of effect of non-toxic level of oridonin on the increase in the sensitivity of HONE 1 to mitoxantrone. Methods HONE 1 was treated with different concentrations of oridonin to acquire non-toxic concentrations of oridonin by MTT essay, and then side population of HONE 1 was detected with the treatment of non- toxic oridonin. Results 5μmol/L of or/donin had no cytotoxieity on HONE1 cells, and the side population phenotype of HONE1 reduced from 3.32% to 0.55% with this concentration of oridonin for 24 hours. 5 μmol/L of oridonin sensitized the different concentrations of mitoxantrone （P〈0.05）. ABCG2 protein decreased on cell membrane. Conclusion Oridonin potentiated the inhibitory effect of mitoxantrone to HONE1 cells by inhibiting the efflux function of ABCG2,

关 键 词：冬凌草甲素 HONE1 米托蒽醌 

分 类 号：R739.63[医药卫生—肿瘤]