β-半乳糖苷酶基因(lacZ)在大型经济海藻裙带菜中的瞬间表达  

Transient Expression of lacZ Reporter Gene in the Economic Seaweed Undaria pinnatifida

作　　者：于道展[1] 秦松[1] 孙国琼[1] 曾呈奎[1] 

机构地区：[1]中国科学院海洋研究所

出　　处：《高技术通讯》2002年第8期93-95,共3页Chinese High Technology Letters

摘　　要：用高压氦气式基因枪将SV4 0启动子驱动下的 β 半乳糖苷酶基因 (lacZ)导入大型经济海藻裙带菜不同部位的组织切块中 ,4 8小时后染色检测 ,在假根、孢子叶和叶片中均检测到了lacZ的瞬间表达。研究还发现 ,对于裙带菜组织块的基因枪法转化 ,压强130 0psi优于 110 0psi。另外在裙带菜中没有发现半乳糖苷酶的染色本底。本文结果提示 :基因枪法是有效的裙带菜遗传转化方法 ;lacZ可以作为裙带菜基因工程研究的报告基因 ,SV4 0启动子能有效驱动外源基因在裙带菜中表达 ,没有组织特异性。这是lacZ在裙带菜中表达的首次报道 。The β-galactosidase gene (lacZ) followed by SV40 promoter was introduced into excised rhizoids, blades and sori of the economic seaweed Undaria pinnatifida using Biolistic PDS-1000/He microjectile bombardment system Transient expression of lacZ was observed in rhizoids, blades and sori by histochemical staining 48 hours after transformation No LacZ activity was observed in either negative or blank controls The result also reveals that microjectile bombardment method is an effective method of Undaria transformation while the bombardment pressure at 1300psi has better transformation effect than that at 1100psi These results suggest that lacZ gene can be used as a useful reporter gene in genetic engineering of Undaria and that SV40 promoter can be an effective promoter without tissue specificity

关 键 词：Β-半乳糖苷酶基因 大型经济海藻 裙带菜 LACZ SV40启动子 基因枪法 瞬间表达 培育 基因工程 

分 类 号：S968.4[农业科学—水产养殖]