布拉酵母与蜡样芽孢杆菌混合固体发酵的培养基优化研究  

Optimization of Fermentation Medium of Saccharomyces boulardii and Bacillus cereus Mixed Solid Fermentation

作　　者：宋平[1] 张靓[1] 赵宝顶 陈二路 张怡[1] 童应凯[1] 

机构地区：[1]天津农学院农学与资源环境学院生物技术系,天津300384

出　　处：《天津科技》2017年第6期61-64,共4页Tianjin Science & Technology

基　　金：天津农学院科学研究发展基金计划"布拉酵母高密度发酵及其在畜牧养殖业中的应用";项目编号2014N14

摘　　要：通过在布拉酵母与蜡样芽孢杆菌混合固体发酵中加入不同量的营养物质进行正交实验,分析产物中布拉酵母菌数,通过对正交实验的结果分析,确定了最佳的配方。以玉米粉、豆饼粉、麸皮的混合培养基为底料,加入4%,葡萄糖、2%,蛋白胨、0.005%,硫酸镁、0.05%,磷酸二氢钾后再加入2,m L布拉酵母与蜡样芽孢杆菌混合菌液,在30,℃培养48,h的条件下,布拉酵母的产量最多。结果显示,以基本饲料成分为底料加入一定速效营养物质,可以得到含有较高数量布拉酵母的饲料添加剂。Through adding different amounts of nutrients to the mixed solid fermentation of Saccharomyces boulardii and Bacillus cereus, the study analyzed the number ofSaccharomyces boulardii in the product. Based on orthogonal experiment results, the optimum formula was determined. With corn powder, soybean powder, wheat bran mixed medium as the bottom material, adding 4% glucose, 2% peptone, 0.005% MgSO4, 0.05% KH2PO4, accessing 2 mL mixed bacterial suspension of Saccharomyces boulardii and Bacillus cereus, cultured at 30 ℃for 48 h, the most number of Saccharomy- ces boulardii was obtained. The results show that on the basis of feed composition as base material, adding a certain available nutrients, a feed additive containing a large quantity of Saccharomyces boulardii can be obtained.

关 键 词：布拉酵母 蜡样芽胞杆菌培 养基优化 

分 类 号：Q939.99[生物学—微生物学]