沉默MDR1基因增强SGC7901/ADM细胞对姜黄素的敏感性  

Silencing MDR1 Gene Enhances the Sensibility of SGC7901/ADM Cells to Curcumin

作　　者：孙新迪[1] 邵淑丽[1] 恽冬泽[1] 李旭艳[1] 张伟伟[1] 付博[1] 李妍[1] 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,齐齐哈尔161006

出　　处：《基因组学与应用生物学》2017年第6期2486-2490,共5页Genomics and Applied Biology

基　　金：黑龙江省自然科学基金(批准号No.C201241;No.C200624);黑龙江省教育厅科学技术项目(批准号12511611)和黑龙江省教育厅基本业务专项理工重点项目(批准号135109104)共同资助

摘　　要：本研究利用短发夹RNA(sh RNA)沉默SGC7901/ADM细胞MDR1基因表达,增强人胃癌SGC7901/ADM细胞对姜黄素的敏感性。根据MDR1基因序列设计3对编码sh RNA的DNA模板,克隆到p Silencer 3.1-H1 neo(p3.1)上构建3种sh RNA表达载体,转染SGC7901/ADM细胞,q RT-PCR和Western blotting检测MDR1基因沉默效果,用荧光显微镜观察细胞形态,MTT法检测细胞活力。结果显示,3种sh RNA表达载体转染细胞后均能不同程度沉默MDR1基因的表达,增强了细胞对姜黄素的敏感性。This dissertation researches some questions about using short hairpin RNA （shRNA） to silence MDR1 gene expression of cells SGC7901/ADM to enhance the sensibility of human gastric cancer cells SGC7901/ADM towards curcumin. According to MDR1 gene sequence, three pairs of DNA template which encoding shRNA had been designed, and clone them to pSilencer 3. 1-H1 neo （p3.1） to construct three kinds of shRNA expression vectors. After that, the SGC7901/ADM were transfected, and Western blotting was applied to detect the silence effects of MDR1 gene, fluorescence microscope was used to observe cell morphology and MTT method was adopted to detect the viability of cells SGC7901/ADM. The results showed that cells, transfected by these three shRNA expression vector, could silence the expression of MDR1 gene in different degrees, and could enhance the sensibility cells of SGC7901/ADM towards curcumin.

关 键 词：SGC7901/ADM细胞 MDR1 SHRNA 姜黄素 

分 类 号：R735.2[医药卫生—肿瘤]