胃癌组织microRNA表达谱检测及生物信息学分析  

Expression profile of microRNAs and bioinformatics analysis in human gastric cancer tissues

作　　者：黄幼生[1] 解娜[1] 张艺馨[1] 罗志飞[1] 薛逢贵 

机构地区：[1]海南医学院第一附属医院病理科,海口570102

出　　处：《临床与实验病理学杂志》2017年第6期591-595,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(81260321)

摘　　要：目的探讨微小RNA(microRNA,miRNA)在胃癌组织中的表达特征。方法采用Exiqon miRNA芯片检测3对胃癌组织及其配对正常胃黏膜组织中miRNA差异表达情况,随机选取10个上调或下调差异表达的miRNA应用qPCR技术验证;应用生物信息学分析差异表达的miRNA及其靶向基因在胃癌发生、发展中的作用。结果芯片结果显示,在3对胃癌组织中有111个miRNA出现差异表达(差异倍数>2,P<0.05),其中上调表达95个,下调表达16个。进一步行qPCR验证的10个miRNA的表达情况与miRNA芯片结果一致(P=0.040)。聚类分析显示胃癌组织与癌旁组织存在明显的差异表达,基因本体(gene ontology,GO)及pathway分析显示差异表达的miRNA涉及胃癌的凋亡、周期转换、分化、侵袭、转移及各种肿瘤通路的激活等。结论胃癌组织中miRNA存在异常表达,可能涉及胃癌的发生、发展。Purpose This experiment was designed to in- vestigate the expression profile of miRNAs （microRNA） in human gastric cancer tissues. Methods The expression profiles of miRNAs were compared between 3 pairs of GC and adjacent norreal tissues using an Exiqon miRNA array, following which quantitative PCR （qPCR） was employed to confirm the results of the miRNA array, and 10 miRNAs were selected. Bioinforrnatics was used to analyze the biological function of the differentially expressed miRNAs and its target genes in gastric cancer. Results Compared with adjacent mucosal tissues, 95 miRNAs were up-regulated and 16 miRNAs were down-regulated in GC （ 〉 2 folds, P 〈 0. 05 ）. The qPCR results were consistent with microarray-based expression analysis （ P = 0. 049 ）. Furthermore, the online GO and pathway analysis revealed that miRNAs might involve RNA transcription, RNA metabolism, gene expression, gene silencing, and other biological functions in GC. Conclusion There is abnormal expression of miRNAs in gastric cancer, and the abnormal expression of miRNAs may be related to GC tumorigenesis.

关 键 词：胃肿瘤 MIRNA 差异表达 生物信息学 MIRNA芯片 

分 类 号：R735.2[医药卫生—肿瘤]