人喉鳞状细胞癌中CMIP的表达及意义  

Clinical significance of CMIP expression in human laryngeal squamous cell carcinoma

作　　者：韦党军 高潮兵[3] 吴正升[1] 

机构地区：[1]安徽医科大学病理学教研室,合肥230032 [2]安徽省阜阳市太和县中医院耳鼻喉科,阜阳236600 [3]安徽医科大学第一附属医院耳鼻喉科,合肥230032

出　　处：《临床与实验病理学杂志》2017年第6期649-652,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：安徽省学术和技术带头人及后备人选科研基金资助项目(2016H074);安徽省高校优秀青年人才支持计划重点项目(gxyq ZD2016046)

摘　　要：目的探讨CMIP(C-Maf inducing protein)基因在人喉鳞状细胞癌(laryngeal squamous cell carcinoma,LSCC)和喉息肉组织中的表达及其临床意义。方法应用实时荧光定量PCR法检测28例人LSCC和23例喉息肉组织中CMIP mRNA的表达;应用免疫组化法检测86例人LSCC和29例喉息肉组织中CMIP蛋白的表达,分析其与LSCC临床病理特征及其预后的关系。结果 LSCC CMIP mRNA和蛋白表达均显著高于喉息肉组织;CMIP表达与LSCC淋巴结转移和临床分期呈显著正相关,并且CMIP表达均与LSCC患者无复发生存期(relapse-free survival,RFS)和总生存期(overall survival,OS)密切相关。结论 CMIP可能参与人LSCC的发生、发展,CMIP表达提高可能是LSCC进展和预后不良的潜在标志物。Purpose To investigate the expression of CMaf inducing protein（CMIP） in human laryngeal squamous cell carcinoma and polyps tissues, and to analyze their association with clinicopathological parameters and survival of patients with laryngeal squamous cell carcinoma. Methods Real-time PCR assay was used to detect the expression of CMIP mRNA in 28 ea- ses of laryngeal squamous cell carcinoma and 23 cases of polyp fresh tissue specimen. Immunohistoehemical staining was used to detect the expression of CMIP in 86 cases of formalin-fixed and parrffin-embedded laryngeal squamous cell carcinoma and 29 cases of polyp tissue specimens. The clinicopathological and prognostic significance of CMIP expression was investigated in laryngeal squamous cell carcinoma. Results The expression of CMIP mRNA and protein was both significantly increased in laryngeal squamous cell carcinoma compared with polyp tissue specimens. The expression of CMIP was significantly associated with tumor lymph node metastasis and late clinical stage of laryngeal squamous cell carcinoma. Furthermore, the expression of CMIP was significantly correlated with poor relapse-free survival and overall survival in laryngeal squamous cell carcinoma. Conclusion CMIP might play an important role in the development and progression of laryngeal squamous cell carcinoma and increased expression of CMIP might imply disease progression and poor outcome in patient with laryngeal squamous cell carcinoma.

关 键 词：喉肿瘤 鳞状细胞癌 CMIP 实时荧光定量PCR 免疫组织化学 

分 类 号：R739.6[医药卫生—肿瘤]