八种食药用菌子实体醇提物对乳腺癌细胞MCF-7的体外影响  

Effect of Ethanolic Extracts from Eight Kinds of Medicinal and Edible Fungi on Breast Cancer Cell MCF-7 in vitro

作　　者：于荣利[1] 贾薇[1] 张劲松[1] 杨焱[1] 吴迪[1] 唐传红[1] 颜梦秋[1] 庄海宁[1] 汪雯翰[1] 

机构地区：[1]上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海市农业遗传育种重点开放实验室,上海201403

出　　处：《食用菌学报》2017年第2期88-92,共5页Acta Edulis Fungi

基　　金：上海市农业科学院青年人才成长计划[沪农青字(2016)第1-14号];上海市农业科学院科技发展基金[农科发2012(08)]资助

摘　　要：以乳腺癌细胞株MCF-7为实验对象,采用细胞增殖抑制率、细胞粘附抑制率、细胞克隆数和细胞迁移距离为评价指标,研究8种食药用菌醇提物对乳腺癌的影响。刺芹侧耳(Pleurotus eryngii)、姬松茸(Agaricus blazei)和灰树花(Grifola frondosa)的醇提物(200μg/mL)处理能显著降低MCF-7细胞的克隆形成数和细胞迁移距离,较阴性对照分别降低了64.3%、64.5%、61.6%和54.8%、54.8%、48.0%,3种醇提物对MCF-7细胞粘附数和细胞增殖的抑制率分别为48.5%、55.1%、49.3%和30.0%、34.1%、29.5%;另外5种食用菌醇提物作用效果不明显。结果初步表明,刺芹侧耳、姬松茸和灰树花的醇提物(200μg/mL)具有一定的体外抑制乳腺癌活性。Effect of ethanolic extracts from medicinal and edible fungi （eight kinds） was evaluated on the breast cancer cell line, MCF-7, by detecting number of cell colon, inhibition rate of cell adherence, cell migration distance and inhibition rate of cell proliferationcell. Experimental data showed that pretreatments with 200 μg/mL Pleurotus eryngii, Agaricus blazei and Grifola frondosa extracts could significantly reduce clonal formation by 64.3%, 64.5%, 61.6% and cell migration distance by 54. 8%, 54. 8%, 48. 0%, respectively, compared with negative control. Their inhibitory effects on the adhesion and cell proliferation were at the rate of 48.5%, 55.1%, 49.3% and 30.0%, 34.1%, 29.5%, respectively. But no significant effect on the four parameters of cells was recorded for other five tested extracts compared with negative control. Our data revealed that among 8 kinds of fungi evaluated in this study, only P. citrinopileus, A. blazei and G. frondosa showed anti-breast cancer activity in vitro.

关 键 词：MCF-7细胞 细胞增殖 粘附能力 细胞迁移 细胞克隆 

分 类 号：R285[医药卫生—中药学]