雌激素受体"对心肌梗死小鼠的保护作用及其机制  

Protective effect and mechanism of estrogen receptor β on myocardial infarction mice

作　　者：姚新亮[1] 万琪琳[1] 张蕾[1] 钟晓鸣[1] 闫成芸 

机构地区：[1]河南大学淮河医院心内科,河南开封475000

出　　处：《东南大学学报（医学版）》2017年第3期373-378,共6页Journal of Southeast University(Medical Science Edition)

基　　金：河南省科技发展计划项目(152300410065)

摘　　要：目的:探讨雌激素受体β(ERβ)对心肌梗死小鼠的保护作用及其机制。方法:取ERβ转基因小鼠(设为Tg-ERβ组)、非转基因的同窝对照野生型C57小鼠(NLC组)、Tg-ERβ转基因心肌梗死小鼠(Tg-ERβ+CAL组)和非转基因的同窝对照野生型C57心肌梗死小鼠(NLC+CAL组),于术后1、3和7 d超声心动图观察小鼠心脏结构和功能;RT-PCR检测小鼠心脏Ⅰ型胶原蛋白、!平滑肌肌动蛋白(α-SMA)和转化生长因子-β(TGF-β)mRNA;Masson染色检测心脏纤维化。结果:与NLC组相比,NLC+CAL组的舒张期左心室后壁厚度(PWTS)、收缩末期左心室前壁厚度(AWTD)、舒张期左心室前壁厚度(AWTS)、射血分数(EF)和短轴缩短率(FS)降低(P<0.05),收缩期左室内径(LVESD),Ⅰ型胶原蛋白、α-SMA和TGF-β增高(P<0.05);与Tg-ERβ组相比,Tg-ERβ+CAL组的AWTD降低,EF、FS、Ⅰ型胶原蛋白、α-SMA和TGF-β增加(P<0.05);与NLC组相比,Tg-ERβ组的LVESD和舒张期左室内径(LVEDD)增高(P<0.05),EF和FS降低(P<0.05);与NLC+CAL组比,Tg-ERβ+CAL组的EF和FS增高(P<0.05),Ⅰ型胶原蛋白mRNA、α-SMA mRNA和TGF-βmRNA明显降低(P<0.05);与Tg-ERβ+CAL组比,NLC+CAL组小鼠心脏冠脉以及心肌间质胶原纤维面积明显增加(P<0.05)。结论:心肌细胞限制性过表达ERβ可通过抑制心脏重塑、心肌纤维化或瘢痕化起到保护梗死心肌的作用。Objective： To explore the protective effect and mechanism of estrogen receptor β（ERβ） on myocardial infarction mice.Methods： The transgenic ERβ mice（Tg-ERβ group）, non-transgenic wild-type C57 mice（NLC group）, Tg-ERβ transgenic myocardial infarction mice（Tg-ERβ＋CAL） and NLC myocardial infarction mice（NLC＋CAL group） were collected into our study.The cardiac structure and function were assessed by echocardiography 1 day, 3 days and 7 days after operation of myocardial infarction.RT-PCR was used to determine the type Ⅰ collagen mRNA, alpha smooth muscle actin（α-SMA） mRNA and transforming growth factor-β（TGF-β） mRNA and Masson staining was employed to detect the cardiac fibrosis.Results： Compared with the NLC group, the PWTS, AWTD, AWTS, EF and FS had decreased（P＆lt;0.05）, while the VESD, type Ⅰ collagen, α-SMA and TGF-β had increased（P＆lt;0.05） in the NLC＋CAL group.Compared with the Tg-ERβ group, AWTD had decreased（P＆lt;0.05）,but the EF, FS, type Ⅰ collagen, α-SMA and TGF-β had increased（P＆lt;0.05） in the Tg-ERβ＋CAL group.Compared with the NLC group, LVESD and LVEDD had increased（P＆lt;0.05）,and the EF and FS had decreased （P＆lt;0.05） in the Tg-ERβgroup.Compared with NLC＋CAL group, EF and FS had increased（P＆lt;0.05）, type Ⅰ collagen, but the α-SMA and TGF-β had decreased（P＆lt;0.05） in the Tg-ERβ＋CAL group.Compared with Tg-ERβ＋ CAL group, the area of interstitial collagen fibers in the coronary artery and myocardial interstitium was increased（P＆lt;0.05） in the NLC＋CAL group.Conclusion： Restricted overexpression of ERβ in cardiomyocytes can protect the myocardium by inhibiting cardiac remodeling and myocardial fibrosis or scarring.

关 键 词：心肌梗死 小鼠 雌激素受体 超声心动图 

分 类 号：R-33[医药卫生] R542.22