SATB2在口腔鳞癌中的表达及对细胞增殖的影响  

Expression of SATB2 in oral squamous cell carcinoma and its role in promoting cell poliferation

作　　者：高洁[1,2] 詹甜甜 葛昕[1,2] 徐增琦 王晨星[1,2] 李怀奇[1,2] 吴煜农[1,2] 叶金海[1,2] 

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室,江苏南京210029 [2]南京医科大学附属口腔医院口腔颌面外科,江苏南京210029

出　　处：《口腔生物医学》2017年第2期75-79,104,共6页Oral Biomedicine

基　　金：国家自然科学基金资助项目(30801301;81371123);江苏省"青蓝工程"项目;"科教强卫工程"医学重点人才项目;江苏高校优势学科建设工程资助项目(2014-37)

摘　　要：目的:检测核基质蛋白特异AT序列结合蛋白2(SATB2)在口腔鳞癌组织中的表达,探讨其对口腔鳞癌细胞增殖的影响。方法:采用实时定量RT-PCR和Western blot分别检测口腔鳞癌组织及HN4细胞系中SATB2的基因和蛋白的表达情况;采用免疫荧光染色检测SATB2在HN4细胞系中的分布情况;通过转染慢病毒的方法过表达SATB2后,CCK-8实验检测其对口腔鳞癌细胞增殖能力的影响,流式细胞术检测细胞周期,Western blot检测细胞周期相关蛋白P63、Cyclin B1及细胞内STAT3磷酸化水平;构建裸鼠荷瘤实验模型,观察其对移植瘤生长的影响。结果:SATB2在口腔鳞癌组织及HN4细胞系中呈高表达,而在癌旁组织和人口腔角质细胞中呈低表达(P<0.05);SATB2基因过表达后显著促进口腔鳞癌细胞的增殖能力,上调细胞周期相关蛋白P63、Cyclin B1,细胞内STAT3磷酸化明显上升,促进体内移植瘤的生长(P<0.05)。结论:SATB2在口腔鳞癌组织及细胞系中表达升高,过表达SATB2能促进肿瘤细胞的增殖能力。Objective To investigate expression of special AT-rich sequence-binding protein 2（SATB2） in oral squamous cell car-cinoma （OSCC）tissue samples,and then to study the proliferation roles of SATB2 in OSCC cells. Methods： The expression of SATB2 in OSCC and HN4 cell lines are tested by Western blot and qRT-PCR,cellular immunofluorescence was perfor^ned to further character-ize the subcellular distribution of SATB2 in HN4 cell lines. Using lentivirus overexpressed SATB2, in vitro, cell proliferation were as-sessed by CCK-8 experiments and the cell cycles were measured by flow cytometry, the protein change of cell cycle-related protein P63,Cyclin B1 and STAT3 phosphor^^lation were tasted by Western blot. In vivo,the growth of tumor transplation with HN4 cells and Lv-SATB2-HN4 cells in nude mice was further observed. Results ： The expression of SATB2 in OSCC and HN4 cell lines are tested by Western blot and qRT-PCR,and expression of SATB2 in OSCC tissues is higher than para-carcinoma tissues and expression of SATB2 in HN4 cells is higher than the oral keratin forums cells （P〈0.05）. CCK-8 assay,flow cytometry and xenograft model showed that over-expression SATB2 in HN4 cell lines significantly promoted cell proliferation,cell cycle-related protein P63,Cyclin B1 had been signifi-cantly up-regulated and STAT3 phosphor^^lation obviously enhanced. Conclusions ： SATB2 was highly expressed both in OSCC tissues and HN4 cell lines. Either in vitro or in vivo ,overexpression of SATB2 promoted cell proliferation.

关 键 词：SATB2 口腔鳞癌 HN4细胞 增殖 

分 类 号：R739.8[医药卫生—肿瘤]