Th17细胞免疫应答对高危型人乳头瘤病毒感染宫颈疾病的影响  

The immune response of Th17 cells in the cervical diseases with high-risk human papillomavirus infection

作　　者：薛纪森[1] 朱华[1] 章圣辉[2] 吕杰强[3] 胡燕[1] 

机构地区：[1]温州医科大学附属第一医院妇科,浙江温州325015 [2]温州医科大学附属第一医院血液内科,浙江温州325015 [3]温州医科大学附属第二医院妇科,浙江温州325027

出　　处：《温州医科大学学报》2017年第7期504-509,共6页Journal of Wenzhou Medical University

基　　金：浙江省卫生厅科研基金资助项目(2011KYA107)

摘　　要：目的:探讨Th17细胞及其主要效应因子IL-17所介导的细胞免疫应答在高危型人乳头瘤病毒(HPV)感染引起宫颈疾病中的作用。方法:收集宫颈癌组(28例)、CIN 3组(29例)、CIN 2组(30例)、CIN 1组(26例)、宫颈高危HPV感染组(25例)以及对照组(30例)血标本。采用流式细胞术检测各组患者外周血中Th17细胞的表达水平,采用ELISA法检测各组患者外周血清中IL-17的表达水平。结果:随宫颈病变的进展,外周血中Th17细胞亚群所占CD4+T细胞比例与对照组比较有逐渐增加的变化趋势,组间Th17细胞表达率的差异有统计学意义(P<0.05)。随着宫颈疾病的进展,各组外周血清中IL-17的表达水平有逐渐升高的变化趋势,差异有统计学意义(P<0.05),但两两比较结果提示各组间差异无统计学意义(P>0.05)。结论:Th17细胞免疫应答及其主要效应因子IL-17与高危型HPV感染引起宫颈疾病的发生、发展相关。Objective： To study the role of cellular immunity in patients of cervical cancer with high-risk human papillomavirus （HPV） infection mediated by Th17 cells and IL-17. Methods： The study groups included 28 cervical cancer patients, 29 CIN3 patients, 30 CIN2 patients, 26 CIN1 patients, 25 high-risk HPV infected healthy women and 30 patients with HPV negative. The percentage of Th17 cells in the peripheral blood was detected by flow cytometry. The concentration of IL-17 in serum was examined by ELISA. Results： During the disease progression of cervical lesions, the proportion of Th17 cells in the total CD4＋ cells showed a gradually in-creased tendency compared with the controls. The proportions differed significantly among the study groups and the control group （P〈0.05）. During the disease progression of cervical lesions, levels of IL-17 in serum showed a gradually increased tendency and differed significantly among all groups （P〈0.05）, but comparisons between any two groups were no significant difference （P〉0.05）. Conclusion： This study indicates that the cellular immuno-reaction mediated by Th17 cells and IL-17 may be related to the infection of high-risk HPV, which contributes to the disease progression of its associated cervical diseases.

关 键 词：Th17细胞 白细胞介素17 宫颈疾病 人乳头瘤病毒 T细胞免疫 

分 类 号：R737.33[医药卫生—肿瘤]