利用固定床反应器进行脂肪酶催化合成维生素A棕榈酸酯  

Synthesis of Vitamin a Palmitate Catalyzed by Lipase in Fixed Bed Reactor

作　　者：林旺锦 姚传义[1] 卢英华[1] 

机构地区：[1]厦门大学化学化工学院,福建厦门361005

出　　处：《泉州师范学院学报》2017年第2期21-25,共5页Journal of Quanzhou Normal University

基　　金：厦门市科技计划项目(3502Z20143008)

摘　　要：探讨了利用固定床反应器进行脂肪酶催化合成维生素A棕榈酸酯的可行性.利用HPD826吸附法获得固定化酶活力达到9 200U/g,确定了反应介质为石油醚,进料流量8mL/min,反应温度为35℃,维生素A乙酯及棕榈酸的浓度分别为3.6g/L和14g/L.在此优化条件下反应,转化率可达80%,维生素A棕榈酸酯的生产能力为281g/(L·h).反应器连续运行12h,转化率维持在68%以上.结果表明,利用固定床反应器进行酶促合成维生素A棕榈酸酯可行,可进一步深入研究.The feasibility of using fixed bed reactor for synthesis of vitamin A palmitate catalyzed by li- pase was investigated in this work.First, the lipase was immobilized by adsorption with HPD826 as car- rier and give the activity of 9200 U/g.Then the appropriate reaction conditions were determined：the re- action media is petroleum ether,the feed flow rate is 8 mL/min,the temperature is 35 ℃ ,the concen- trations of vitamin A acetate and palmitic acid are 3.6 and 14 g/L.Under these conditions,the conver- sion and productivity of vitamin A palmitate can reach 80% and 281 g/（L · h） ,respectively.Finally,the reactor was continuously run for 12 h,the conversion held upon 68%.These results showed that the synthesis of vitamin A palmitate by immobilized lipase in fixed bed reactor was feasible and worth fur- ther investigation.

关 键 词：固定床 脂肪酶 固定化 有机相 维生素A棕榈酸酯 

分 类 号：TQ033[化学工程]