苏丹红I对小鼠卵巢的损伤作用及对Caspase-3和Ki-67表达的影响  

Injury and Expression of Caspase-3 and Ki-67 in Ovary of Mice Exposed to Sudan I

作　　者：魏明[1] 甘露[2] 龙卫红[2] 侯进[1] 邓雅婷[1] 陈玉龙[3] 陈丽宏[2] 

机构地区：[1]西安医学院药理毒理学教研室,西安710021 [2]陕西省人民医院妇科,西安710068 [3]西安医学院基础与转化医学研究所,西安710021

出　　处：《生态毒理学报》2017年第2期88-93,共6页Asian Journal of Ecotoxicology

基　　金：陕西省自然科学基础研究项目(2016JM8046;2014JQ2-8055);西安医学院校级重点建设学科资助项目(2015097);陕西省卫计委重点项目(2014A);陕西省教育厅重点科研攻关计划项目(16JS096);西安医学院2016博士科研启动基金项目(2016DOC22)

摘　　要：苏丹红是一种人工合成偶氮染料,可引起肝脏及泌尿系统等多个脏器的肿瘤,但苏丹红对生殖系统的毒性研究较少。本研究以昆明小鼠为受试对象,探讨苏丹红I对小鼠卵巢组织Caspase-3和Ki-67表达的影响。将24只昆明小鼠随机分为:对照组、低剂量(60 mg·kg^(-1))、中剂量(120 mg·kg^(-1))、高剂量(240 mg·kg^(-1))4组,6只/组。胃灌4周,4周末处死。HE染色观察各组卵巢组织病理变化,免疫组化法检测卵巢组织Caspase-3和Ki-67表达,Real time PCR检测卵巢组织Caspase-3和Ki-67 m RNA表达,Western blotting检测卵巢组织中Ki-67及Caspase-3蛋白表达。与对照组比较,实验组卵巢中Caspase-3阳性细胞率显著升高(P<0.05),而Ki-67阳性细胞率显著降低(P<0.05),Real time PCR及Western blotting结果与免疫组化法结果一致。本实验发现苏丹红染毒后颗粒细胞的Caspase-3表达增加,且Caspase-3表达呈剂量依赖性,但Ki-67表达受到抑制。Sudan is a synthetic azo dye and can cause tumors in multiple organs including liver and urinary system, but the research in reproductive system toxicity is rare. The effect of Sudan I on the expression of Caspase-3 and Ki-67 in the ovarian tissue of mice was studied by gastric gavage. The 24 Kunming mice were randomly divid- ed into 4 groups： control group, low dose （60 mg·kg-1）, medium dose （120mg·kg-1） and high dose （240 mg·kg-1） group. Each group contained 6 mice. After gastric gavage for 4 weeks, the pathological changes were observed by HE staining. The expression of Caspase-3 and Ki-67 in ovary was detected by immunohistochemistry and the mR- NA expression and protein expression of Ki-67 and Caspase-3 in mouse ovarian tissues were detected by real time PCR and western blotting. Compared with the control group, the rate of Ki-67 positive cells was significantly lower in high concentration group （P〈0.05）. The rate of Caspase-3 positive cells was significantly increased （P〈0.05） in high concentration group and the results of PCR and western blotting were consistent with the immunohistochemical results. The Caspase-3 expression of ovarian granulosa cell was increased after SDI exposure, but the Ki-67 expression was inhibited.

关 键 词：苏丹红I 卵巢 CASPASE-3 KI-67 

分 类 号：X171.5[环境科学与工程—环境科学]