P16和SOX2蛋白在子宫颈活检上皮内瘤变中的诊断意义  

Significance of P16 and SOX2 protein in the diagnosis of cervical intraepithelial neoplastic

作　　者：桂华伟[1] 王绪明[2] 黄利华[1] 黄萱[2] 陈琼霞[2] 刘丽江[2] 

机构地区：[1]武汉市普爱医院病理科,湖北武汉430056 [2]江汉大学江大病理诊断所组织病理部

出　　处：《中国实验诊断学》2017年第6期942-945,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助项目(No.81272754)

摘　　要：目的研究P16、P53、SOX2及Ki-67在子宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)病变中的表达及临床价值分析。方法收集子宫颈活检病例92例,用免疫组化染色检查P16、P53、SOX2及Ki-67在子宫颈CIN病变表达中的表达情况,分析其与CIN分级的相关性。结果 P16蛋白的表达与分布与CIN形态学分级之间有较好的一致性。SOX2阳性细胞在鳞状上皮中的分布与CIN的分级趋向一致。P53蛋白和Ki-67指数与CIN的分级趋向一致。结论在临床实践中,对于子宫颈小样本活检的CIN的分级,将HE染色的形态学观察与P16、P53、SOX2及Ki-67的免疫组化检查相结合,可获得比较客观的明确诊断,且重复性较好。Objective To study the relationship between the expression of P16, P53, SOX2 and Ki67 and cervtcal CIN lesion. Methods To examine the expression of P16,P53,SOX2 and Ki-67 in 92 cases of cervical CIN lesions by immunohistochemistry,and analyze the correlation between the expression of and CIN classification. Results There was a good agreement between the expression and distribution of P16 protein and the morphological classification of CIN. The distribution of SOX2 positive cells in the squamous epithelium was consistent with the CIN classification. The clear relationship between P53 protein and Ki-67 index and CIN morphological classification has also been shown. Con- clusion The combination of immunohistochemical examination of hematoxylin and eosin staining of morphological ob servation and P16 and P53 and SOX2 and Ki-67 get clear diagnosis more objective and repeatable in clinical practice for the classification of cervical small biopsy samples of CIN.

关 键 词：子宫颈活检 上皮内瘤变 P16 P53 SOX2 KI67 

分 类 号：R737.33[医药卫生—肿瘤]