OsmC基因在豌豆根瘤菌抗氧化和共生中的功能  

Antioxidative and Symbiosis Function of OsmC gene in Rhizobium leguminosarum

作　　者：程国军[1] 彭杨[1] 殷杰[1] 田梦洋[1] 

机构地区：[1]中南民族大学生命科学学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2017年第2期25-29,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：湖北省自然科学基金资助项目(2014CFB914);中央高校基本科研业务费专项(CZW15112)

摘　　要：为研究OsmC基因在豌豆根瘤菌抗氧化和共生固氮中的功能,以豌豆根瘤菌RL3841的OsmC基因为研究对象,通过同源重组获得了OsmC基因突变株RLOsmC.结果表明:OsmC基因突变不影响豌豆根瘤菌的自生生长能力,但对无机氧化物H_2O_2和低浓度有机氧化物氢过氧化枯烯(CuOOH)都十分敏感.突变株RLOsmC感染豌豆宿主能形成红色有效根瘤,但是其固氮酶活降低了18.3%.OsmC基因的表达不受H_2O_2的诱导,但在7 d根瘤类菌体中表达量显著上调.表明根瘤菌OsmC基因在共生和抗氧化功能中发挥了重要的作用.To study the antioxidative and symbiosis nitrogen fixation function of OsmC gene in Rhizobium leguminosarum, OsmC mutant RLOsmC was constructed by homologous recombination.The results indicated that mutation of OsmC gene had no effect on the self-growth ability of Rhizobium leguminosarum, but it was significantly sensitive to both H2O2 and cumene hydroperoxyde （CuOOH） with low concention.Red effective nodules could be formed when mutant strains RLOsmC infected host peas, but the capacity of nitrogenase was reduced by 18.3%.OsmC gene expression could not be induced by H2O2, while its expression was markedly increased in 7 d nodule bacteroids.Overall, it was revealed that rhizobial OsmC gene played an important role in symbiotic and antioxidative function.

关 键 词：豌豆根瘤菌 OsmC基因 抗氧化功能 共生功能 实时荧光定量RT-PCR 

分 类 号：Q935[生物学—微生物学]