PRV感染PK-15细胞对prv-miR-LLT11a表达时相的影响  

Effects on Temporal Expression of prv-miR-LLT11a in PK-15 Cells Infected by Pseudorabies Virus

作　　者：杨利[1] 石昂 李新果[1] 李双双[1] 时庆贺 郑关民[1] 王玉国[1] 陈陆[1] 王川庆[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《河南农业科学》2017年第6期120-124,共5页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31272567);河南省高校科技创新人才支持计划项目(14HASTIT022);河南省高校科技创新团队支持计划项目(14IRTSTHN015)

摘　　要：为研究伪狂犬病病毒(PRV)的致病机制,将PRV QBA株按0.5个感染复数(MOI)的剂量接种PK-15细胞,分别在感染后0、1、2、4、6、8、12、24 h收取细胞并提取microRNA(miRNA),采用茎环引物实时荧光定量PCR(RT-q PCR)检测prv-miR-LLT11a的表达时相。RT-q PCR扩增结果显示,熔解曲线峰值单一,扩增曲线拐点清晰。RT-q PCR检测结果表明,PRV QBA株感染PK-15细胞1 h后,prv-miR-LLT11a表达量极显著升高,随后表达量下调,在感染6 h后表达量降至最低,感染8 h后表达量持续急剧升高。In order to study the pathogenic mechanism of pseudorabies virus （PRY） , PK-15 cells were in -fected with PRY QBA strain at 0. 5 M0I,and cells were harvested and microRNA（ miRNA） were extrac-ted at 0,1 ,2 ,4,6,8,12 ,24 hours post infection（ hpi）. Then the temporal expression of prv-miR-LLTl 1 a was detected by stem-loop real-time quantitative PCR （ RT-qPCR） . The amplification results of RT-qPCR showed that the melting curve had a single peak and a clear inflection point. RT-qPCR data showed that the expression of prv-miR-LLTl 1 a remarkably significant increased in PK-15 cells after 1 hour infection with PRY QBA strain, then decreased. Its expression level decreased to the lowest level at 6 hpi, and in-creased significantly after 8 hpi.

关 键 词：伪狂犬病病毒 prv-miR-LLT11a 茎环RT-q PCR 表达时相 

分 类 号：S855.3[农业科学—临床兽医学]