纤维堆囊菌So ce2161限制修饰系统研究  

The Preliminary Study on the Restriction and Modification System in Sorangium Cellulosum So ce2161

作　　者：孙宝婷 祝文兴[1] 刘新利[1] 

机构地区：[1]齐鲁工业大学生物工程学院,济南250353

出　　处：《齐鲁工业大学学报》2017年第3期21-24,共4页Journal of Qilu University of Technology

基　　金：国家自然科学基金项目(21272139)

摘　　要：采用双向电泳技术从So ce2161中未获得内源性质粒,通过对So ce2161培养液、细胞壁及菌体内部DNA核酸酶进行提取纯化,并与外源基因孵育,发现了有较强活性的DNA核酸酶,对DH5α基因组DNA、质粒pET-32a均有降解作用。In this paper,two dimensional electrophoresis are used to obtain the endogenous plasmid from ce2161,but there is no endogenous plasmid in the bacteria. The nuclease inside and outside the bacteria is found by analyzing the nuclease activity in fermentation, on the bacteria cell wall and in the bacteria. DH5a genomic DNA and plasmid pET-32a were degraded by the nuclease.

关 键 词：纤维堆囊菌 核酸酶 限制修饰系统 

分 类 号：TQ927[轻工技术与工程—发酵工程]