CRISPR-Cas9系统敲除甜瓜ACC合成酶基因表达载体的构建  

Construction of Expression Vector Knocking Out ACC Synthase Gene in Melon by CRISPR-Cas9 System

作　　者：王雪[1] 李冠[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《北方园艺》2017年第12期114-118,共5页Northern Horticulture

摘　　要：以甜瓜品种"老汉瓜"为试材,采用CRISPR-Cas9基因组编辑技术,研究了构建CRISPR-Cas9系统敲除甜瓜ACC合成酶基因表达载体的方法,以期获得高效的敲除甜瓜基因的基因组编辑技术。结果表明:以甜瓜ACC合成酶的基因设计出引物后,将其构建到pP1C.4载体上,用热激法将连接产物转化到大肠杆菌中,筛选的阳性克隆测序验证为最终载体。The CRISPR-Cas9 genome editing techniques of melon,was constructed a vector which knockout ACC synthase gene by CRISPR-Cas9 system, and then the high efficient genome editing technique was obtained which could knockout melon genes. The results showed that based on the ACC synthase gene of melon to designed primers,and then they were builded to the pPIC. 4 vector. The connect product was transformed into escherichia coli by heat shock method. Finally,a few positive clones were selected,they were final vector.

关 键 词：CRISPR-Cas9 基因敲除 ACC合成酶 

分 类 号：S336[农业科学—作物遗传育种]