反复熔铸对钴铬烤瓷合金细胞相容性的影响  

Cytocompatibility of Co-Cr ceramic alloys after recasting

作　　者：林泓磊[1,2] 江磊[1,2] 张长源[1,2,3] 林东红[4] 程辉[1,2,3] LIN Hong-lei JIANG Lei ZHANG Chang-yuan LIN Dong-hong CHENG Hui(School of Stomatology, Fujian Medical University. Fuzhou 350004 Hospital of Stomatology, Fujian Medical University. Fuzhou 350002 Research Centre of Dental Biomechanics ＆ Aesthetic College of Medical Technology and Engineering, Fujian Medical University. Fuzhou 350004, Fujian Province, China)

机构地区：[1]福建医科大学口腔医学院,福建福州350004 [2]福建医科大学附属口腔医院,福建福州350002 [3]福建医科大学口腔生物力学及美学研究中心 [4]福建医科大学医学技术与工程学院,福建福州350004

出　　处：《上海口腔医学》2017年第3期246-250,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81371174);福建省自然科学基金(2016J01354);福建省医学创新课题(2015-CX-29);福建省卫生计生委青年科研课题(2015-2-15)

摘　　要：目的 :探讨反复熔铸对钴铬烤瓷合金生物相容性的影响。方法 :将钴铬烤瓷合金分别经过1~3次熔铸,使用DMEM细胞培养液进行浸提并采用电感耦合等离子体原子发射光谱仪(ICP-AES),检测浸提液中金属离子的种类和水平,使用合金浸提液培养小鼠肺结缔组织成纤维细胞(L-929),采用流式细胞仪检测L-929细胞周期的改变,Annexin-V-FITC/PI法检测L-929细胞凋亡与死亡情况。采用SPSS 13.0软件包对数据进行统计学分析。结果 :经过反复熔铸后,第3代合金与第1、第2代相比,除镓(Ga)的析出量增高外(P<0.05),其他金属离子的析出量及金属离子析出的总量均无显著变化(P>0.05);第1~3代合金对L-929细胞周期的影响无显著差异,早期凋亡率、晚期凋亡与坏死率差异均无显著性(P>0.05)。结论:经1~3次熔铸的钴铬烤瓷合金生物相容性未发生明显改变。PURPOSE： To evaluate the cytocompatibility of Co-Cr ceramic alloys after recasting. METHODS： Cobalt- chromium （Co-Cr） ceramic alloys were recasted for 1-3 times. Samples of Co-Cr ceramic alloys were immersed in DMEM culture medium. The concentration of ions of leaching liquor was determined by ICP-MS total quantization method. L-929 cells were cultured with leaching liquor, flow cytometry was used to test cell cycle progression, Annexin-V-FITC/PI apoptosis staining kit was used to quantitatively detect cell death patterns. SPSS 13.0 software package was used for statistical analysis. RESULTS： After casting for 1-3 times, no significant increase in the concentration of ions of Co-Cr ceramic alloys was noted （P〉0.05） except Ga （P〈0.05） . No significant differences in L-929 cell cycle were found in Co- Cr ceramic alloys between the samples cast for 1 time and for 2-3 times （P〉0.05）. No significant difference in the rates of apoptosis and necrosis was found （P〉0.05）. CONCLUSIONS： The biocompatibility of Co-Cr ceramic alloys casted for 1 - 3 times is not changed.

关 键 词：钴铬烤瓷合金 反复熔铸 离子析出 细胞周期 细胞凋亡 

分 类 号：R783.1[医药卫生—口腔医学]